Invitrogen

COX2 Polyclonal Antibody

COX2 Antibody in Immunocytochemistry (ICC/IF)

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COX2 Antibody (PA5-16817) in ICC/IF

Immunofluorescence analysis of COX2 was performed using 70% confluent log phase HT-29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with COX2 Rabbit Polyclonal Antibody (PA516817) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution o... View More

COX2 Antibody in Immunohistochemistry (IHC)

COX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

COX2 Antibody — Endothelial cell-specific Cox-2-knockout in Cox-2flox/flox;VECadCreERT2Tg mice. (A) Polymerase chain reaction detection of Cox-2flox recombination. Mice were injected with tamoxifen (five successive days) to activate CreERT2. Endothelial cells were enriched with anti-LSEC antibody-conjugated beads. Genomic DNAs were isolated from flow-through cells and the endothelial cell-enriched antibody-bead bound fractions and analyzed by polymerase chain reaction to detect Cox-2flox (2067 bp) and Cox-2del (651 bp) alleles. Lanes 1 and 2: Cox-2flox/flox cells. Lanes 3 and 4: Cox-2flox/flox;VECadCreERT2Tg cells. Lanes 1 and 3: flow-through fraction; lanes 2 and 4: bound fraction. (B) Peritoneal macrophage genomic DNA analyzed by Southern blotting. Cox-2flox (9.0 kb) and Cox-2del (8.0 kb) alleles are present in Cox-2flox/del heterozygous macrophages (lane 1). The Cox-2del allele is absent in Cox-2flox/flox;VECadCreERT2Tg macrophages (lane 2). (C) Absence of COX-2 expression in endothelial cells of DSS-treated Cox-2flox/flox;VECadCreERT2Tg mice. Colon sections from Cox-2flox/flox;VECadCreERT2Tg and Cox-2flox/flox littermates treated with 2.5% DSS for 8 days were costained with anti-COX-2 (red) and anti-CD34 (endothelial cell marker, green). Double-positive cells are observed on blood vessel structures in the colons of littermate Cox-2flox/flox mice (arrowheads). In contrast, fewif anyCD34-positive cells express COX-2 in Cox-2flox/flox;VECadCreERT2Tg colons. (D) Colon sections similar to t {KO}

Product Details

PA5-16817

Applications

Tested Dilution

Publications

Western Blot (WB)

2-4 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 14 publications 14 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to c-terminus of rat COX2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4°C

Shipping conditions

Wet ice

AB_10983557

Product Specific Information

PA5-16817 targets COX2 in IHC (P), IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.

The PA5-16817 immunogen is synthetic peptide corresponding to c-terminus of rat COX2.

Target Information

COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). It is constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and is up-regulated under pathological conditions, such as in cancer and inflammation (in contrast to the iso-enzyme PTGS1, which is expressed ubiquitously). Up-regulation of COX2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, COX2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. COX2 is naturally inhibited by calcitriol (the active form of Vitamin D). Glucocorticoids chronically trans-repress PTGS2 gene activity in vivo in part by interfering with transcription initiation and elongation. COX2 is a target of NSAID such as aspirin, which can reduce pain and swelling from inflammation driven by COX2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Antagonizing pathways leading to differential dynamics in colon carcinogenesis in Shugoshin1 (Sgo1)-haploinsufficient chromosome instability model.

Molecular carcinogenesis

Rao CV,Sanghera S,Zhang Y,Biddick L,Reddy A,Lightfoot S,Dai W,Yamada HY

PA5-16817 was used in immunohistochemistry - paraffin section to assess the use of mitotic error-induced CIN and Shugoshin1 haplo insufficient mice as models to study chromosome instability in colon cancer

Sun May 01 00:00:00 UTC 2016

Aspirin blocks growth of breast tumor cells and tumor-initiating cells and induces reprogramming factors of mesenchymal to epithelial transition.

Laboratory investigation; a journal of technical methods and pathology

Maity G,De A,Das A,Banerjee S,Sarkar S,Banerjee SK

PA5-16817 was used in western blot to evaluate the effect of aspirin in mesenchymal to epithelial transition

Wed Jul 01 00:00:00 UTC 2015

PharmDB-K: Integrated Bio-Pharmacological Network Database for Traditional Korean Medicine.

PloS one

Lee JH,Park KM,Han DJ,Bang NY,Kim DH,Na H,Lim S,Kim TB,Kim DG,Kim HJ,Chung Y,Sung SH,Surh YJ,Kim S,Han BW

PA5-16817 was used in western blot to present PharmDB-K, a database offering comprehensive information relating to TKM-associated drugs, disease indication, and protein relationships

Mon Jun 13 00:00:00 UTC 2016

Genistein improves 3-NPA-induced memory impairment in ovariectomized rats: impact of its antioxidant, anti-inflammatory and acetylcholinesterase modulatory properties.

PloS one

Menze ET,Esmat A,Tadros MG,Abdel-Naim AB,Khalifa AE

PA5-16817 was used in immunohistochemistry - paraffin section to characterize how 3-NPA-induced memory impairment in ovariectomized rats is improved by Genistein and its anti-inflammatory, antioxidant, and acetylcholinesterase modulatory properties

Fri Nov 20 00:00:00 UTC 2015

Comparative immunohistochemical assessment of cutaneous cyclooxygenase-2 enzyme expression in chronological aging and photoaging.

Photodermatology, photoimmunology & photomedicine

Habib MA,Salem SA,Hakim SA,Shalan YA

PA5-16817 was used in immunohistochemistry to perform a comparative study of the immunohistochemical expression of COX-2 in skin samples from individuals with chronologically or photo aged skin

Sat Feb 01 00:00:00 UTC 2014

Immunohistochemical evaluation of COX-1 and COX-2 expression in keloid and hypertrophic scar.

The American Journal of dermatopathology

Abdou AG,Maraee AH,Saif HF

PA5-16817 was used in immunohistochemistry to evaluate COX-1 and COX-2 expression in keloid and hypertrophic scars

Tue Apr 01 00:00:00 UTC 2014

Therapeutic effect of coenzyme Q10 against experimentally-induced hepatocellular carcinoma in rats.

Environmental toxicology and pharmacology

Fouad AA,Al-Mulhim AS,Jresat I

PA5-16817 was used in immunohistochemistry to study the therapeutic effects of coenzyme Q10 in a rat model of hepatocellular carcinoma

Tue Jan 01 00:00:00 UTC 2013

Simvastatin treatment ameliorates injury of rat testes induced by cadmium toxicity.

Biological trace element research

Fouad AA,Albuali WH,Jresat I

PA5-16817 was used in immunohistochemistry to study the mechanisms underlying the protection against cadmium-induced testicular toxicity conferred by treatment with simvastatin

Sat Jun 01 00:00:00 UTC 2013

The effects of ulipristal on Bax/Bcl-2, cytochrome c, Ki-67 and cyclooxygenase-2 expression in a rat model with surgically induced endometriosis.

European journal of obstetrics, gynecology, and reproductive biology

Huniadi CA,Pop OL,Antal TA,Stamatian F

PA5-16817 was used in immunohistochemistry to use a rat model of endometriosis to study the effects of ulipristal on endometrial histology and the expression of Bax/Bcl-2, cytochrome c, Ki-67 and COX-2

Mon Jul 01 00:00:00 UTC 2013

A promise in the treatment of endometriosis: an observational cohort study on ovarian endometrioma reduction by N-acetylcysteine.

Evidence-based complementary and alternative medicine : eCAM

Porpora MG,Brunelli R,Costa G,Imperiale L,Krasnowska EK,Lundeberg T,Nofroni I,Piccioni MG,Pittaluga E,Ticino A,Parasassi T

PA5-16817 was used in immunohistochemistry to study the efficacy of N-acetylcysteine therapy in women with ovarian endometriosis

Thu Jul 04 00:00:00 UTC 2013

Suppression of β-catenin and cyclooxygenase-2 expression and cell proliferation in azoxymethane-induced colonic cancer in rats by rice bran phytic acid (PA).

Asian Pacific journal of cancer prevention : APJCP

Saad N,Esa NM,Ithnin H

PA5-16817 was used in immunohistochemistry to study the ability of phytic acid from rice bran to reduce the expression of beta-catenin and COX-2 and to inhibit colon cancer proliferation in a rat carcinogen-induced model

Tue Oct 14 00:00:00 UTC 2014

Multimechanistic antifibrotic effect of biochanin a in rats: implications of proinflammatory and profibrogenic mediators.

PloS one

Breikaa RM,Algandaby MM,El-Demerdash E,Abdel-Naim AB

PA5-16817 was used in immunohistochemistry to assess the antifibrotic of biochanin A

Wed Feb 19 00:00:00 UTC 2014

Reappraisal of the therapeutic role of celecoxib in cholangiocarcinoma.

PloS one

Yeh CN,Chiang KC,Juang HH,S Pang JH,Yu CS,Lin KJ,Yeh TS,Jan YY

PA5-16817 was used in immunohistochemistry to evaluate the efficacy and safety of celecoxib to treat cholangiocarcinoma

Tue Apr 29 00:00:00 UTC 2014

Protective effects of nebivolol against cold restraint stress-induced gastric ulcer in rats: role of NO, HO-1, and COX-1,2.

Nitric oxide : biology and chemistry

Morsy MA,Heeba GH,Abdelwahab SA,Rofaeil RR

PA5-16817 was used in immunohistochemistry to study the protective mechanism of nebivolol in a rat gastric ulcer model

Wed Aug 15 00:00:00 UTC 2012

Role of immunohistochemical cyclo-oxygenase-2 (COX-2) and osteocalcin in differentiating between osteoblastomas and osteosarcomas.

The Malaysian journal of pathology

El-Badawi ZH,Muhammad EM,Noaman HH

PA5-16817 was used in immunohistochemistry to study the differentiation of osteoblastomas and osteosarcomas based on the immunohistochemical measurement of COX-2 and osteocalcin

Fri Jun 01 00:00:00 UTC 2012

Resveratrol suppresses 4-hydroxyestradiol-induced transformation of human breast epithelial cells by blocking IκB kinaseβ-NF-κB signalling.

Free radical research

Park SA,Na HK,Surh YJ

PA5-16817 was used in western blot to study the role of IKK-beta and NFkB in the mechanism by which resveratrol blocks human breast epithelial transformation induced by 4-hydroxyestradiol

Wed Aug 01 00:00:00 UTC 2012

Inhibition of COX-2 in colon cancer modulates tumor growth and MDR-1 expression to enhance tumor regression in therapy-refractory cancers in vivo.

Neoplasia (New York, N.Y.)

Rahman M,Selvarajan K,Hasan MR,Chan AP,Jin C,Kim J,Chan SK,Le ND,Kim YB,Tai IT

PA5-16817 was used in immunohistochemistry and western blot to study the beneficial effects on tumor growth and MDR-1 expression of pharmacological COX-2 inhibition in chemoresistant colorectal cancer

Sun Jul 01 00:00:00 UTC 2012

Cyclooxygenase-2 expression and its prognostic significance in clear cell renal cell carcinoma.

Korean journal of pathology

Lee JW,Park JH,Suh JH,Nam KH,Choe JY,Jung HY,Chae JY,Moon KC

PA5-16817 was used in immunohistochemistry and western blot to study the prognostic value of the immunohistochemical expression of COX-2 in patients with clear cell renal cell carcinoma

Fri Jun 01 00:00:00 UTC 2012

The acid sphingomyelinase inhibitors block interferon-α-induced serotonin uptake via a COX-2/Akt/ERK/STAT-dependent pathway in T cells.

International immunopharmacology

Su HC,Ma CT,Lin CF,Wu HT,Chuang YH,Chen LJ,Tsao CW

PA5-16817 was used in western blot to study the role of sphingomyelinase inhibitors in serotonin uptake

Tue Nov 01 00:00:00 UTC 2011

Cox-2 deletion in myeloid and endothelial cells, but not in epithelial cells, exacerbates murine colitis.

Carcinogenesis

Ishikawa TO,Oshima M,Herschman HR

PA5-16817 was used in immunohistochemistry to investigate the involvement of COX2 expression in different cell types in the pathogenesis of colitis

Tue Mar 01 00:00:00 UTC 2011

Cyclooxygenase (COX)-1 activity precedes the COX-2 induction in Aβ-induced neuroinflammation.

Journal of molecular neuroscience : MN

Dargahi L,Nasiraei-Moghadam S,Abdi A,Khalaj L,Moradi F,Ahmadiani A

PA5-16817 was used in western blot to investigate the activation of cyclooxygenases in the neuroinflammation mediated by Abeta

Thu Sep 01 00:00:00 UTC 2011

Tumor formation in a mouse model of colitis-associated colon cancer does not require COX-1 or COX-2 expression.

Carcinogenesis

Ishikawa TO,Herschman HR

PA5-16817 was used in immunohistochemistry to investigate the influence of COX-1 or COX-2 on specific tumor formation in mice

Thu Apr 01 00:00:00 UTC 2010

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COX2 Polyclonal Antibody

COX2 Antibody (PA5-16817) in ICC/IF

Product Details

PA5-16817

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

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