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Invitrogen
CHRM2 Polyclonal Antibody
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FIGURE: 1 / 2
CHRM2 Antibody (PA5-111845) in IHC (F)
Immunohistochemistry analysis of CHRM2 in frozen mouse parieto-temporal cortex sections tissue sections using CHRM2 Antibody (Product # PA5-111845) at a dilution of 1:100. mAChR M2 staining (red) was dense in layer IV, with fibers climbing to layers II-III. Kir3.2 K+ channel staining (green) was dense in layers IV and I. Overlapping expression of Kir3.2 channel and mAChR M2 is seen in cortical layers.
Product Details
PA5-111845
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
GST fusion protein corresponding to amino acid residues 225-356 of human M2
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2857254
Product Specific Information
Reconstitute antibody with 50 µL of double distilled water (DDW). The reconstituted solution can be stored at 4 degrees C for up to 1 week. For longer periods, small aliquots should be stored at -20 degrees C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
GST fusion protein sequence is: VANQDPVSPS LVQGRIVKPN NNNMPSSDD GLEHNKIQNG KAPRDPVTEN CVQGEEKESS NDSTSV SAV ASNMRDDEIT QDENTVSTSL GHSKDENSKQ TCIRIGTKTP KS DSCTPTN TTVEVVGSSG QNGDE
Homology: Chimpanzee, gorilla amino acid residues identical; orangutan - 130/132 amino acid residues identical; pig - 122/132 amino acid residues identical; rat - 118/132 amino acid residues identical; mouse - 117/132 amino acid residues identical; dog - 122/132 amino acid residues identical.
Target Information
Muscarinic cholinergic receptor 2 (CHRM2) is an Acetylcholine Receptor has been suggested to function as a presynaptic autoreceptor that inhibits acetylcholine release. The M2 receptor has also been suggested to mediate smooth muscle contractility. Alzheimer's disease (AD) involves selective loss of M2 subtype receptors in cortical and hippocampal regions of the human brain. Increase in M2 receptor heterozygosity has been associated with depression in women. The M2 receptor has been reported to be expressed in various regions of the brain including the cerebellum, cerebral cortex, hippocampus, medulla, striatum, and thalamus, and in lung and prostate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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