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Invitrogen
CDK9 Polyclonal Antibody
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FIGURE: 1 / 1
CDK9 Antibody (PA1-84581) in WB
Western blot analysis of CDK9 was performed by loading 50 µg of J-lat full (10.6) whole cell lysate in reducing sample buffer onto a 4-15% tris-glycine gel. Proteins were transferred to PVDF and then blocked in blocking buffer (TBST+5% non-fat milk) for one hour at room temperature. CDK9 was detected using an anti-CDK9 polyclonal antibody (Product # PA1-84581) at a dilution of 1:1,000 overnight at 4°C on a rocking platform. Goat anti Rabbit HRP-conjugated secondary antibody was diluted at 1:2000 in 1xTBST buffer with 5% non fat milk and in... View More
Product Details
PA1-84581
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Multiple synthetic peptides corresponding to the C-terminal and N-terminal domains of human CDK9.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage conditions
4°C or -20°C if preferred
Shipping conditions
Wet ice
RRID
AB_2077305
Product Specific Information
PA1-84581 does not cross-react with other cyclin dependent kinases.
Rabbit anti Human Cdk9 antibody recognizes a approximately 43 kDa member of the cyclin-dependent protein kinase (CDK) family, CDK9, previously named PITALRE.
Target Information
CDK9 is a serine/threonine kinase which when complexed with CyclinT1 forms an active holoenzyme complex. This complex plays a key role in regulating the eukaryotic cell cycle. CDK9 is coexpressed and copurified with CyclinT1. CDK9 regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors. CDK9 promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. The CDK9/cyclin-K complex also has kinase activity towards CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 causes cardiac myocyte enlargement leading to cardiac hypertrophy and confers a predisposition to heart failure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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