Invitrogen

CD45RA Monoclonal Antibody (OX-33)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers
CD45RA Monoclonal Antibody (OX-33)

Product Details

MA1-81427

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent
-

Flow Cytometry (Flow)

1:50 -1:100
View 4 publications 4 publications
Product Specifications

Species Reactivity

Rat

Published species

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OX-33

Immunogen

Purified rat spleen leucocyte common antigen.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_928455

Product Specific Information

MA1-81427 is directed against a high molecular weight band of the leucocyte common antigen (LCA). This clone only labels B-cells among thoracic duct lymphocytes, demonstrating low reactivity with bone marrow and no reactivity with thymocytes.

PLP fixation is recommended for optimal tissue staining results. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Rat CD45RA antibody, clone OX-33 is directed against a high molecular weight band of the leucocyte common antigen.

Target Information

CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD45; L-CA; leucocyte common antigen; Leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; Ly-5; Lyt-4; Protein tyrosine phosphatase, receptor-type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T100; T200; T200 glycoprotein

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Gene Aliases: CD45; L-CA; Lca; Ptprc; RT7; T200

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UniProt ID: (Rat) P04157

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Entrez Gene ID: (Rat) 24699

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Function(s)
protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding heparin binding protein kinase regulator activity protein kinase binding heparan sulfate proteoglycan binding
Process(es)
activation of MAPK activity negative regulation of T cell mediated cytotoxicity positive regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process positive regulation of T cell mediated immunity regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of humoral immune response mediated by circulating immunoglobulin negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration leukocyte cell-cell adhesion response to gamma radiation dephosphorylation B cell differentiation T cell differentiation positive regulation of B cell proliferation negative regulation of protein autophosphorylation heterotypic cell-cell adhesion peptidyl-tyrosine dephosphorylation T cell proliferation B cell proliferation positive regulation of T cell proliferation positive regulation of MAPK cascade cell cycle phase transition positive thymic T cell selection negative thymic T cell selection regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of alpha-beta T cell proliferation positive regulation of isotype switching to IgG isotypes bone marrow development stem cell development negative regulation of peptidyl-tyrosine phosphorylation T cell receptor signaling pathway B cell receptor signaling pathway regulation of B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway
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