Invitrogen

CD3/CD4/CD8a Antibody Cocktail, APC, FITC, PerCP-eFluor™ 710, eBioscience™

4 References

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

22-0306-71

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

20 µL/test
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Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Class

Cocktail

Type

Antibody

Clone

SK3, SK7, SK1

Conjugate

APC , FITC , PerCP-eFluor™ 710 APC , FITC , PerCP-eFluor™ 710 APC , FITC , PerCP-eFluor™ 710

Form

Liquid

Concentration

20 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10671078

Product Specific Information

Description: This three-color cocktail is designed to identify T cells from peripheral blood samples. It can be used alone, or in combination with other antibodies to further characterize T cell populations. The SK3 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The SK7 monoclonal antibody reacts with human CD3 eplison, a 20 kDa subunit of the CD3/TCR complex. The CD3 complex, including the epsilon, gamma and delta subunits, is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The SK1 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8beta chain (CD8alpha beta) or as a homodimer (CD8alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a.

This cocktail contains the following antibodies:

Anti-Human CD4 (SK3) FITC

Anti-Human CD3 (SK7) PerCP-eFluor™ 710

Anti-Human CD8a (SK1) APC

Applications Reported: eZfluor Anti-Human CD4 FITC, CD3 PerCP-eFluor™ 710, CD8a APC has been reported for use in flow cytometric analysis.

Applications Tested: eZfluor Anti-Human CD4 FITC, CD3 PerCP-eFluor™ 710, CD8a APC has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL per test, which contains 0.06 µg of Anti-Human CD4 FITC, 0.06 µg of Anti-Human CD3 PerCP-eFluor™ 710, and 0.25 µg of Anti-Human CD8a APC. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Target Information

CD8 (Cluster of Differentiation 8) is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediate efficient cell-cell interactions within the immune system. The CD8 antigen acts as a co-receptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 is found on a T cell subset of normal cytotoxic/suppressor cells which make up approximately 20-35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer (NK) cells, subpopulations of peripheral blood null cells, thymocytes and bone marrow cells. The CD8 co-receptor functions as either a homodimer composed of two alpha chains, or as a disulfide-linked heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. The majority of CD8+ T cells express CD8 as a alpha/beta heterodimer. In HIV, the HIV-2 envelope glycoprotein binds CD8 alpha chain (but not the beta chain).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 epsilon; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; FLJ18683; Leu-2; Leu2 T-lymphocyte antigen; OKT3, delta chain; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen; TCR zeta chain

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Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; CD4; CD4mut; CD8; CD8A; CD8B; CD8B1; IMD17; IMD18; IMD19; IMD25; LEU2; LY3; LYT3; MAL; p32; P37; T3D; T3E; T3G; T3Z; TCRE; TCRZ

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UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693, (Human) P01730, (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917, (Human) 920, (Human) 925, (Human) 926

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Function(s)
transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding virus receptor activity glycoprotein binding receptor activity extracellular matrix structural constituent zinc ion binding coreceptor activity immunoglobulin binding enzyme binding MHC class II protein binding protein tyrosine kinase binding MHC class I protein binding
Process(es)
positive regulation of T cell anergy protein complex assembly cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway signal complex assembly G-protein coupled receptor signaling pathway response to nutrient positive regulation of gene expression negative regulation of gene expression T cell costimulation positive regulation of interferon-gamma production positive regulation of interleukin-4 production positive regulation of T cell proliferation T cell activation regulation of apoptotic process negative thymic T cell selection positive regulation of interleukin-2 biosynthetic process negative regulation of smoothened signaling pathway positive regulation of alpha-beta T cell proliferation positive regulation of peptidyl-tyrosine phosphorylation regulation of immune response positive regulation of calcium-mediated signaling T cell receptor signaling pathway apoptotic signaling pathway T cell differentiation positive thymic T cell selection positive regulation of transcription from RNA polymerase II promoter adaptive immune response immune response regulation of defense response to virus by virus T cell mediated immunity antigen processing and presentation cytotoxic T cell differentiation defense response to virus cytokine production induction by virus of host cell-cell fusion signal transduction enzyme linked receptor protein signaling pathway positive regulation of calcium ion transport into cytosol fusion of virus membrane with host plasma membrane entry into host cell response to estradiol maintenance of protein location in cell response to vitamin D helper T cell enhancement of adaptive immune response T cell selection positive regulation of protein kinase activity defense response to Gram-negative bacterium regulation of T cell activation viral process Fc-gamma receptor signaling pathway involved in phagocytosis establishment or maintenance of cell polarity protein transport regulation of lymphocyte apoptotic process
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