Invitrogen

CD25 Monoclonal Antibody (eBio3C7 (3C7)), Functional Grade, eBioscience™

5 Published Figures

12 References

View all (228) CD25 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (5)

FIGURE: 1 / 5

CD25 Antibody (16-0253-85) in Flow

Nature communications 2019 - Fig. 7 Increased Tregs in XO ki tumors. Tumors were resected 12 days after inoculation and enzymatically digested. a , b Graphs and FACS profiles indicating the proportions of CD4+Foxp3+ cells, Gr-1+ neutrophils and F4/80+ macrophages in tumors from WT, XDH ki, and XO ki mice. Results are expressed as mean +- SEM from 5 mice. c Representative FACS profiles and quantification of CD4+CD25+Foxp3+ T cells in vi... View More

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Product Details

16-0253-85

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 9 publications 9 publications

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

In vitro Assay (IV)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio3C7 (3C7)

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2573074

Product Specific Information

Description: The eBio3C7 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of the IL-2 receptor is inhibited by the 3C7 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody and subsequent detection with another CD25 antibody.

Applications Reported: This eBio3C7 (3C7) antibody has been reported for use in flow cytometric analysis and functional activity.

Applications Tested: This eBio3C7 (3C7) antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. (Fluorochrome-conjugated eBio3C7 (3C7) is recommended for use in flow cytometry).

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

IL-1β-MyD88-mTOR Axis Promotes Immune-Protective IL-17A⁺Foxp3⁺ Cells During Mucosal Infection and Is Dysregulated With Aging.

Frontiers in immunology

Bhaskaran N,Faddoul F,Paes da Silva A,Jayaraman S,Schneider E,Mamileti P,Weinberg A,Pandiyan P

16-0253-85 was used in Flow Cytometry, In vitro Assay to identify IL-1β/MyD88/Treg axis as a new component that modulates inflammatory responses in oral mucosa.

Mon Jun 28 00:00:00 UTC 2021

IL-1β-MyD88-mTOR Axis Promotes Immune-Protective IL-17A⁺Foxp3⁺ Cells During Mucosal Infection and Is Dysregulated With Aging.

Frontiers in immunology

Bhaskaran N,Faddoul F,Paes da Silva A,Jayaraman S,Schneider E,Mamileti P,Weinberg A,Pandiyan P

16-0253-85 was used in Flow Cytometry, In vitro Assay to identify IL-1β/MyD88/Treg axis as a new component that modulates inflammatory responses in oral mucosa.

Mon Jun 28 00:00:00 UTC 2021

Targeted knock-in mice expressing the oxidase-fixed form of xanthine oxidoreductase favor tumor growth.

Nature communications

Kusano T,Ehirchiou D,Matsumura T,Chobaz V,Nasi S,Castelblanco M,So A,Lavanchy C,Acha-Orbea H,Nishino T,Okamoto K,Busso N

Published figure using CD25 monoclonal antibody (Product # 16-0253-85) in Flow Cytometry

Mon Oct 28 00:00:00 UTC 2019

miR-451 limits CD4⁺ T cell proliferative responses to infection in mice.

Immunologic research

Chapman LM,Ture SK,Field DJ,Morrell CN

16-0253 was used in Flow cytometry/Cell sorting to demonstrate that miR-451 is a regulator of host immune responses to Plasmodium infection, particularly in the control of CD4+ T cell proliferation via a Myc-dependent pathway.

Tue Aug 01 00:00:00 UTC 2017

Transforming growth factor-β1 sustains the survival of Foxp3(+) regulatory cells during late phase of oropharyngeal candidiasis infection.

Mucosal immunology

Bhaskaran N,Quigley C,Weinberg A,Huang A,Popkin D,Pandiyan P

16-0253 was used in Flow cytometry/Cell sorting to investigate how CD4(+)CD25(+)Foxp3(+) Tregs are controlled during mouse oropharyngeal candidiasis infection.

Fri Jul 01 00:00:00 UTC 2016

Co-potentiation of antigen recognition: A mechanism to boost weak T cell responses and provide immunotherapy in vivo.

Science advances

Hoffmann MM,Molina-Mendiola C,Nelson AD,Parks CA,Reyes EE,Hansen MJ,Rajagopalan G,Pease LR,Schrum AG,Gil D

16-0253 was used in Flow cytometry/Cell sorting to investigate co-potentiation, showing that weak T-cell responses can be boosted to provide immunotherapy in vivo.

Thu Oct 01 00:00:00 UTC 2015

MFG-E8 Is Critical for Embryonic Stem Cell-Mediated T Cell Immunomodulation.

Stem cell reports

Tan Y,AlKhamees B,Jia D,Li L,Couture JF,Figeys D,Jinushi M,Wang L

Published figure using CD25 monoclonal antibody (Product # 16-0253-85) in Flow Cytometry

Tue Nov 10 00:00:00 UTC 2015

Inflammation enhances IL-2 driven differentiation of cytolytic CD4 T cells.

PloS one

Workman AM,Jacobs AK,Vogel AJ,Condon S,Brown DM

16-0253 was used in Flow cytometry/Cell sorting to show that IL-2 signalling was necessary for induction of an immune response during influenza A infection.

Tue Jan 27 00:00:00 UTC 2015

RIP1-driven autoinflammation targets IL-1α independently of inflammasomes and RIP3.

Nature

Lukens JR,Vogel P,Johnson GR,Kelliher MA,Iwakura Y,Lamkanfi M,Kanneganti TD

16-0253 was used in Flow cytometry/Cell sorting to investigate how mutations in SHP-1 causes inflammatory disease, showing that RIP1-driven autoinflammation targets IL-1α independently of inflammasomes and RIP3.

Thu Jun 13 00:00:00 UTC 2013

Heterogeneity of respiratory dendritic cell subsets and lymphocyte populations in inbred mouse strains.

Respiratory research

Hackstein H,Wachtendorf A,Kranz S,Lohmeyer J,Bein G,Baal N

Published figure using CD25 monoclonal antibody (Product # 16-0253-85) in Flow Cytometry

Mon Oct 15 00:00:00 UTC 2012

Efficient induction of CD25- iTreg by co-immunization requires strongly antigenic epitopes for T cells.

BMC immunology

Geng S,Yu Y,Kang Y,Pavlakis G,Jin H,Li J,Hu Y,Hu W,Wang S,Wang B

16-0253 was used in in vitro experiments to investigate the antigenic requirements for the induction of CD25- inducible regulatory T cells.

Thu May 05 00:00:00 UTC 2011

Zoonotic orthopoxviruses encode a high-affinity antagonist of NKG2D.

The Journal of experimental medicine

Campbell JA,Trossman DS,Yokoyama WM,Carayannopoulos LN

16-0253 was used in Blocking experiments to investigate how viruses interfere with the function of major histocompatibility complex class I superfamily (MHCISF) receptors to evade immunologic cytotoxicity, showing that zoonotic orthopoxviruses encode a high-affinity antagonist of NKG2D.

Mon Jun 11 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: CD25; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; il-2 receptor alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R alpha chain; IL-2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; IL2 receptor subunit alpha; IL2-RA; IL2R subunit alpha; Il2ra antibody; interleukin-2 receptor alpha; Interleukin-2 receptor subunit alpha; Interleukin2 receptor subunit alpha; p55; p55 chain; sIL 2R; soluble IL 2 receptor

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Gene Aliases: CD25; Il2r; Il2ra; Ly-43

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UniProt ID: (Mouse) P01590

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Entrez Gene ID: (Mouse) 16184

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Function(s)

interleukin-2 receptor activity protein binding drug binding interleukin-2 binding

Process(es)

inflammatory response to antigenic stimulus activation-induced cell death of T cells inflammatory response immune response Notch signaling pathway interleukin-2-mediated signaling pathway positive regulation of T cell proliferation positive regulation of activated T cell proliferation negative regulation of T cell proliferation T cell homeostasis positive regulation of T cell differentiation regulation of T cell homeostatic proliferation negative regulation of lymphocyte proliferation negative regulation of defense response to virus negative regulation of inflammatory response negative regulation of immune response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD25 Monoclonal Antibody (eBio3C7 (3C7)), Functional Grade, eBioscience™

CD25 Antibody (16-0253-85) in Flow

Product Details

16-0253-85

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

In vitro Assay (IV)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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