Invitrogen

CD207 (Langerin) Monoclonal Antibody (eBioRMUL.2), eBioscience™

9 Published Figures

14 References

View all (38) Langerin (CD207) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (9)

CD207 (Langerin) Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 11

CD207 (Langerin) Antibody (14-2073-82) in IHC

Staining of mouse epidermal sheets using Anti-Mouse CD207 (Langerin) Purified (top) or Rat IgG2a K Isotype Control Purified (Product # 14-4321-82) (bottom). Courtesy of Mark Udey and Chris Nagao.

CD207 (Langerin) Antibody in Western Blot (WB)

CD207 (Langerin) Antibody in Immunocytochemistry (ICC/IF)

CD207 (Langerin) Antibody in Flow Cytometry (Flow)

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Product Details

14-2073-82

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

Assay-Dependent

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-Dependent

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

eBioRMUL.2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_493943

Product Specific Information

Description: RMUL.2 is a monoclonal antibody that reacts with the predicted extracellular domain of mouse CD207, also known as Langerin. Mouse CD207 is expressed in a distinct subset of dendritic cells called Langerhans cells (LC). Mouse CD207 is a 48 kDa, C-type lectin and putative transmembrane protein that likely plays a role in antigen recognition and uptake. LC are located in the epidermis and upon activation reduce expression of CD207 and begin migrating through the dermis towards lymphatic vessels. Expression of mouse CD207 in LC has been correlated to the presence of Birbeck Granules. Furthermore, the formation of Birbeck Granules has been observed upon transfection of CD207 cDNA into fibroblast cell lines.

Western Blotting with RMUL.2 reveals a 48 kDa band in lysate from mouse ear epidermis as well as in lysates from cells transfected with mouse CD207 cDNA. Cross reactivity of this antibody with other proteins has not been determined. Preliminary data from mouse CD207 cDNA transfected cells suggests that RMUL.2 can be used for intracellular staining followed by flow cytometric analysis. We have observed in our transfectants the majority of protein is expressed intracellularly with a small percentage on the cell surface. In addition, RMUL.2 has also been tested by immunohistology on mouse ear epidermal sheets and shows specific staining.

Applications Reported: This eBioRMUL.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining. (Fluorochrome conjugated eBioRMUL.2 is recommended for use in intracellular flow cytometry.).

Applications Tested: This eBioRMUL.2 antibody has been tested by immunoblotting. This can be used at less than or equal to 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Lamgerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Airway Administration of Bacterial Lysate OM-85 Protects Mice Against Respiratory Syncytial Virus Infection.

Frontiers in immunology

Antunes KH,Cassão G,Santos LD,Borges SG,Poppe J,Gonçalves JB,Nunes EDS,Recacho GF,Sousa VB,Da Silva GS,Mansur D,Stein RT,Pasquali C,De Souza APD

14-2073-82 was used in Flow cytometry/Cell sorting to suggest that intranasal use of OM-85 should be considered as a potential prophylactic product to prevent RSV bronchiolitis once human studies confirm these findings.

Tue May 24 00:00:00 UTC 2022

Stat3 activation in epidermal keratinocytes induces Langerhans cell activation to form an essential circuit for psoriasis via IL-23 production.

Journal of dermatological science

Nakajima K,Kataoka S,Sato K,Takaishi M,Yamamoto M,Nakajima H,Sano S

14-2073 was used in Immunohistochemistry (Frozen) to show Stat3 activation in keratinocytes may impact on LC activation in situ and their presence may be essential for the pathogenesis of psoriasis.

Fri Feb 01 00:00:00 UTC 2019

Macrophage migration inhibitory factor protects from nonmelanoma epidermal tumors by regulating the number of antigen-presenting cells in skin.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Brocks T,Fedorchenko O,Schliermann N,Stein A,Moll UM,Seegobin S,Dewor M,Hallek M,Marquardt Y,Fietkau K,Heise R,Huth S,Pfister H,Bernhagen J,Bucala R,Baron JM,Fingerle-Rowson G

14207382 was used in immunohistochemistry to propose a tumor-suppressive activity for macrophage migration inhibitory factor in murine skin

Wed Feb 01 00:00:00 UTC 2017

In vivo quantification of quantum dot systemic transport in C57BL/6 hairless mice following skin application post-ultraviolet radiation.

Particle and fibre toxicology

Jatana S,Palmer BC,Phelan SJ,Gelein R,DeLouise LA

Published figure using CD207 (Langerin) monoclonal antibody (Product # 14-2073-82) in Immunofluorescence

Fri Apr 14 00:00:00 UTC 2017

In vivo quantification of quantum dot systemic transport in C57BL/6 hairless mice following skin application post-ultraviolet radiation.

Particle and fibre toxicology

Jatana S,Palmer BC,Phelan SJ,Gelein R,DeLouise LA

Published figure using CD207 (Langerin) monoclonal antibody (Product # 14-2073-82) in Immunofluorescence

Fri Apr 14 00:00:00 UTC 2017

Single-Cell Transcriptomics Reveals that Differentiation and Spatial Signatures Shape Epidermal and Hair Follicle Heterogeneity.

Cell systems

Joost S,Zeisel A,Jacob T,Sun X,La Manno G,Lönnerberg P,Linnarsson S,Kasper M

14-2073 was used in Immunohistochemistry on paraffin embedded tissues to investigate how cellular heterogeneity of murine telogen epidermis is tuned at the transcriptional level, showing that differentiation and spatial signatures shape epidermal and hair follicle heterogeneity.

Wed Sep 28 00:00:00 UTC 2016

Prevention of ultraviolet radiation‑induced immunosuppression by sunscreen in Candida albicans‑induced delayed‑type hypersensitivity.

Molecular medicine reports

Chen Q,Li R,Zhao X,Liang B,Ma S,Li Z,Zhu H

Fri Jul 01 00:00:00 UTC 2016

Prevention of ultraviolet radiation‑induced immunosuppression by sunscreen in Candida albicans‑induced delayed‑type hypersensitivity.

Molecular medicine reports

Chen Q,Li R,Zhao X,Liang B,Ma S,Li Z,Zhu H

Fri Jul 01 00:00:00 UTC 2016

Langerhans Cells Facilitate UVB-Induced Epidermal Carcinogenesis.

The Journal of investigative dermatology

Lewis JM,Bürgler CD,Freudzon M,Golubets K,Gibson JF,Filler RB,Girardi M

14-2073 was used in Immunohistochemistry-immunofluorescence to study the role of Langerhans cells in UVB-induced cutaneous carcinogenesis.

Sun Nov 01 00:00:00 UTC 2015

Systemic sensitization with the protein allergen ovalbumin augments local sensitization in atopic dermatitis.

Journal of inflammation research

Yoo J,Manicone AM,McGuire JK,Wang Y,Parks WC

14-2073 was used in Immunofluorescence to reveal a mouse model of atopic dermatitis that reveals novel insights into the pathogenesis of this complex disease.

Thu Mar 27 00:00:00 UTC 2014

Langerhans cells serve as immunoregulatory cells by activating NKT cells.

Journal of immunology (Baltimore, Md. : 1950)

Fukunaga A,Khaskhely NM,Ma Y,Sreevidya CS,Taguchi K,Nishigori C,Ullrich SE

14-2073 was used in Immunohistochemistry to investigate the function of Langerhans cells isolated from UV-irradiated mice.

Fri Oct 15 00:00:00 UTC 2010

Antibody to Langerin/CD207 localizes large numbers of CD8alpha+ dendritic cells to the marginal zone of mouse spleen.

Proceedings of the National Academy of Sciences of the United States of America

Idoyaga J,Suda N,Suda K,Park CG,Steinman RM

Published figure using CD207 (Langerin) monoclonal antibody (Product # 14-2073-82) in Immunofluorescence

Tue Feb 03 00:00:00 UTC 2009

Epidermal Langerhans cells promote skin allograft rejection in mice with NF-kappa B-impaired T cells.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Molinero LL,Zhou P,Wang Y,Harlin H,Kee B,Abraham C,Alegre ML

Tue Jan 01 00:00:00 UTC 2008

Selective 5-lipoxygenase expression in Langerhans cells and impaired dendritic cell migration in 5-LO-deficient mice reveal leukotriene action in skin.

The Journal of investigative dermatology

Doepping S,Funk CD,Habenicht AJ,Spanbroek R

14-2073 was used in Immunohistochemistry to examine the role of endogenous leukotrienes in dendritic cell function through the study of 5-lipoxygenase-deficient mice.

Sun Jul 01 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: C-type lectin domain family 4 member K; CD207; Langerhans cell specific c-type lectin; Langerin

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Gene Aliases: Cd207; Clec4k

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UniProt ID: (Mouse) Q8VBX4

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Entrez Gene ID: (Mouse) 246278

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Function(s)

carbohydrate binding

Process(es)

defense response to virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD207 (Langerin) Monoclonal Antibody (eBioRMUL.2), eBioscience™

CD207 (Langerin) Antibody (14-2073-82) in IHC

Product Details

14-2073-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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