Invitrogen

CD207 (Langerin) Monoclonal Antibody (eBioL31), eBioscience™

CD207 (Langerin) Antibody in Western Blot (WB)

FIGURE: 1 / 1

CD207 (Langerin) Antibody (14-2075-82) in WB

Mouse CD207-transfected (Lane 2) and untransfected control HEK-293T cell (Lane 1) lysates were immunoblotted with 1 µg/mL of Anti-Mouse CD207 (Langerin) Purified and visualized with Anti-Rat IgG HRP.

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Product Details

14-2075-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/test

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 8 publications 8 publications

Immunoprecipitation (IP)

Assay-Dependent

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

eBioL31

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_763454

Product Specific Information

Description: The eBioL31 monoclonal antibody reacts with mouse CD207, also known as Langerin, which is expressed in a distinct subset of dendritic cells called Langerhans cells (LC). mouse CD207 is a 48 kDa C-type lectin transmembrane protein that likely plays a role in antigen recognition and uptake. LC are located in the epidermis and upon activation, reduce CD207 expression and begin migrating through the dermis towards lymphatic vessels. Expression of mouse CD207 in LC has been correlated to the presence of Birbeck Granules. Furthermore, the formation of Birbeck Granules has been observed upon transfection of CD207 cDNA into fibroblast cell lines.

Western Blotting with eBioL31 reveals a 48kDa band in lysate from mouse ear epidermis, as well as in lysates from cells transfected with mouse CD207 cDNA. Preliminary data from mouse CD207 cDNA transfected cells suggests that eBioL31 can be used for intracellular flow cytometric analysis in combination with the Foxp3/Transcription Factor Staining Bufer Set (Product # 00-5523). The epitope recognized by eBioL31 is in the extracellular domain within the CRD (carbohydrate recognition domain) and therefore can be used to detect both surface and intracellular langerin. Differences in expression has been observed between mouse strains.

Applications Reported: This eBioL31 antibody has been reported for use in flow cytometric analysis, intracellular staining followed by flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections. (Fluorochrome conjugated eBioL31 is recommended for use in intracellular flow cytometry).

Applications Tested: This eBioL31 antibody has been tested by flow cytometric analysis of transfected cells and western blotting of mouse ear lysates. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Lamgerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transport of antibody into the skin is only partially dependent upon the neonatal Fc-receptor.

PloS one

Nasir G,Sinnis P

14-2075-82 was used in Immunocytochemistry to suggest that FcRn plays a role in the transport of IgG into the skin but is not the major driver of IgG transport into this tissue.

Wed Apr 26 00:00:00 UTC 2023

Fibroblastic niches prime T cell alloimmunity through Delta-like Notch ligands.

The Journal of clinical investigation

Chung J,Ebens CL,Perkey E,Radojcic V,Koch U,Scarpellino L,Tong A,Allen F,Wood S,Feng J,Friedman A,Granadier D,Tran IT,Chai Q,Onder L,Yan M,Reddy P,Blazar BR,Huang AY,Brennan TV,Bishop DK,Ludewig B,Siebel CW,Radtke F,Luther SA,Maillard I

14-2075 was used in Flow cytometry/Cell sorting to reveal what we believe to be a previously unrecognised Notch-mediated immunopathogenic role for stromal cell niches in secondary lymphoid organs after allo-BMT and define a framework of early cellular and molecular interactions that regulate T cell alloimmunity.

Mon Apr 03 00:00:00 UTC 2017

VACCINES. A mucosal vaccine against Chlamydia trachomatis generates two waves of protective memory T cells.

Science (New York, N.Y.)

Stary G,Olive A,Radovic-Moreno AF,Gondek D,Alvarez D,Basto PA,Perro M,Vrbanac VD,Tager AM,Shi J,Yethon JA,Farokhzad OC,Langer R,Starnbach MN,von Andrian UH

14-2075 was used in Flow cytometry/Cell sorting to report that mucosal exposure to ultraviolet light (UV)-inactivated Ct (UV-Ct) generated regulatory T cells that exacerbated subsequent Ct infection.

Fri Jun 19 00:00:00 UTC 2015

Inhibition of the TNF Family Cytokine RANKL Prevents Autoimmune Inflammation in the Central Nervous System.

Immunity

Guerrini MM,Okamoto K,Komatsu N,Sawa S,Danks L,Penninger JM,Nakashima T,Takayanagi H

14-2075-82 was used in Flow Cytometry to show that receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL) on T cells regulates C-C type chemokine ligand 20 (CCL20) production by astrocytes and T cell localization in the CNS.

Tue Dec 15 00:00:00 UTC 2015

Distinct Murine Mucosal Langerhans Cell Subsets Develop from Pre-dendritic Cells and Monocytes.

Immunity

Capucha T,Mizraji G,Segev H,Blecher-Gonen R,Winter D,Khalaileh A,Tabib Y,Attal T,Nassar M,Zelentsova K,Kisos H,Zenke M,Seré K,Hieronymus T,Burstyn-Cohen T,Amit I,Wilensky A,Hovav AH

14-2075 was used in Immunofluorescence in immunohistochemistry on frozen tissues to elucidate the distinct cellular precursors of murine langerhans cells in steady state.

Tue Aug 18 00:00:00 UTC 2015

Keratin-dependent regulation of Aire and gene expression in skin tumor keratinocytes.

Nature genetics

Hobbs RP,DePianto DJ,Jacob JT,Han MC,Chung BM,Batazzi AS,Poll BG,Guo Y,Han J,Ong S,Zheng W,Taube JM,Čiháková D,Wan F,Coulombe PA

14-2075 was used in Flow cytometry/Cell sorting to provide new insight into keratin intermediate filament and Aire function, as well as a molecular basis for the K17-dependent amplification of inflammatory and immune responses in diseased epithelia.

Sat Aug 01 00:00:00 UTC 2015

Antigen-Specific Th17 Cells Are Primed by Distinct and Complementary Dendritic Cell Subsets in Oropharyngeal Candidiasis.

PLoS pathogens

Trautwein-Weidner K,Gladiator A,Kirchner FR,Becattini S,Rülicke T,Sallusto F,LeibundGut-Landmann S

14-2075 was used in Flow cytometry/Cell sorting to provide important new insights to our understanding of tissue-specific antifungal immunity.

Thu Oct 01 00:00:00 UTC 2015

The atypical chemokine receptor ACKR2 suppresses Th17 responses to protein autoantigens.

Immunology and cell biology

Hansell CA,MacLellan LM,Oldham RS,Doonan J,Chapple KJ,Anderson EJ,Linington C,McInnes IB,Nibbs RJ,Goodyear CS

14-2075 was used in Flow cytometry/Cell sorting to investigate the role of ACKR2 in autoimmunity, showing that it suppresses Th17 responses to protein autoantigens.

Sun Feb 01 00:00:00 UTC 2015

BRAF-V600E expression in precursor versus differentiated dendritic cells defines clinically distinct LCH risk groups.

The Journal of experimental medicine

Berres ML,Lim KP,Peters T,Price J,Takizawa H,Salmon H,Idoyaga J,Ruzo A,Lupo PJ,Hicks MJ,Shih A,Simko SJ,Abhyankar H,Chakraborty R,Leboeuf M,Beltrão M,Lira SA,Heym KM,Bigley V,Collin M,Manz MG,McClain K,Merad M,Allen CE

14-2075 was used in Immunofluorescence on frozen tissues to investigate the role of BRAF-V600E mutations in langerhans cell histiocytosis, showing that expression in precursor versus differentiated dendritic cells defines clinically distinct risk groups.

Mon Apr 07 00:00:00 UTC 2014

Second-generation Langerhans cells originating from epidermal precursors are essential for CD8+ T cell priming.

Journal of immunology (Baltimore, Md. : 1950)

Elnekave M,Furmanov K,Shaul Y,Capucha T,Eli-Berchoer L,Zelentsova K,Clausen BE,Hovav AH

14-2075 was used in Immunofluorescence in immunohistochemistry on frozen tissues to indicate that second-generation Ag-expressing Langerhans cells differentiating from epidermal precursors directly prime CD8(+) T cells.

Sat Feb 15 00:00:00 UTC 2014

Intradermal delivery of Shigella IpaB and IpaD type III secretion proteins: kinetics of cell recruitment and antigen uptake, mucosal and systemic immunity, and protection across serotypes.

Journal of immunology (Baltimore, Md. : 1950)

Heine SJ,Diaz-McNair J,Andar AU,Drachenberg CB,van de Verg L,Walker R,Picking WL,Pasetti MF

14-2075 was used in Immunohistochemistry to demonstrate the potential of intradermal vaccination with IpaB and IpaD to prevent Shigella infection.

Sat Feb 15 00:00:00 UTC 2014

Glycosidic Tn-based vaccines targeting dermal dendritic cells favor germinal center B-cell development and potent antibody response in the absence of adjuvant.

Blood

Freire T,Zhang X,Dériaud E,Ganneau C,Vichier-Guerre S,Azria E,Launay O,Lo-Man R,Bay S,Leclerc C

14-2075 was used in Flow cytometry/Cell sorting to study dendritic cell antigen uptake and presentation by targeting the antigen receptor, macrophage galactose-type lectin (MGL), with tumor-associated Tn antigens.

Thu Nov 04 00:00:00 UTC 2010

Fusion proteins for versatile antigen targeting to cell surface receptors reveal differential capacity to prime immune responses.

Journal of immunology (Baltimore, Md. : 1950)

Kratzer R,Mauvais FX,Burgevin A,Barilleau E,van Endert P

14-2075 was used in Functional assays to investigate the relationship between the choice of the targeted receptor and the quality of adaptive immune responses.

Tue Jun 15 00:00:00 UTC 2010

Antibody to Langerin/CD207 localizes large numbers of CD8alpha+ dendritic cells to the marginal zone of mouse spleen.

Proceedings of the National Academy of Sciences of the United States of America

Idoyaga J,Suda N,Suda K,Park CG,Steinman RM

Tue Feb 03 00:00:00 UTC 2009

Antibody to Langerin/CD207 localizes large numbers of CD8alpha+ dendritic cells to the marginal zone of mouse spleen.

Proceedings of the National Academy of Sciences of the United States of America

Idoyaga J,Suda N,Suda K,Park CG,Steinman RM

Tue Feb 03 00:00:00 UTC 2009

Matrilysin (Matrix Metalloproteinase-7) regulates anti-inflammatory and antifibrotic pulmonary dendritic cells that express CD103 (alpha(E)beta(7)-integrin).

The American journal of pathology

Manicone AM,Huizar I,McGuire JK

14-2075 was used in Immunohistochemistry on frozen tissues to assess matrix metalloproteinase-7 effects on CD103-E-cadherin interactions in lung injury.

Tue Dec 01 00:00:00 UTC 2009

Novel subset of CD8{alpha}+ dendritic cells localized in the marginal zone is responsible for tolerance to cell-associated antigens.

Journal of immunology (Baltimore, Md. : 1950)

Qiu CH,Miyake Y,Kaise H,Kitamura H,Ohara O,Tanaka M

14-2075 was used in Immunohistochemistry to indicate that a CD8alpha(+) dendritic cell subset is essential for tolerance induction by apoptotic cell clearance.

Wed Apr 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: C-type lectin domain family 4 member K; CD207; Langerhans cell specific c-type lectin; Langerin

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Gene Aliases: Cd207; Clec4k

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UniProt ID: (Mouse) Q8VBX4

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Entrez Gene ID: (Mouse) 246278

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Function(s)

carbohydrate binding

Process(es)

defense response to virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD207 (Langerin) Monoclonal Antibody (eBioL31), eBioscience™

CD207 (Langerin) Antibody (14-2075-82) in WB

Product Details

14-2075-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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