Invitrogen

CD19 Monoclonal Antibody (771404)

CD19 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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CD19 Antibody (MA5-24372) in IHC (F)

Immunohistochemical analysis of CD19 in perfusion fixed frozen sections of rat spleen. Samples were incubated with CD19 monoclonal antibody (Product # MA5-24372) using a dilution of 25 µg/mL overnight at 4 °C followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to B-cells inside the germinal layers.

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Product Details

MA5-24372

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

8-25 µg/mL

Product Specifications

Species Reactivity

Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

771404

Immunogen

CHO-derived recombinant rat CD19 Met1-Gly287

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2576241

Product Specific Information

Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.

Target Information

CD19 is a member of the immunoglobulin superfamily, characterized by two Ig-like domains, and is expressed on B cells throughout all stages of development, excluding terminally differentiated plasma cells. It is also expressed on follicular dendritic cells and has been observed on myeloid leukemia cells, particularly those of monocytic lineage. CD19 is considered the earliest and broadest B cell-restricted antigen, and its expression is found in all B cell precursor leukemias. CD19 forms a multimolecular complex with CD21, CD81, Leu13, MHC class II, and the B cell receptor (BCR), playing a crucial role in B cell signaling. As a signal-amplifying coreceptor for the BCR, CD19 lowers the threshold for antigen receptor-dependent stimulation, allowing B cells to respond specifically and sensitively to various antigens through low-affinity antigen receptors. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells. Beyond its role as a BCR coreceptor, CD19 can also signal independently of BCR co-ligation, serving as a central regulatory component upon which multiple signaling pathways converge. This makes CD19 an important functional regulator of both normal and malignant B cell proliferation. Mutations in the CD19 gene can result in hypogammaglobulinemia, a condition characterized by low levels of immunoglobulins, while CD19 overexpression can lead to B cell hyperactivity. CD19 is expressed on 100% of peripheral B cells, as defined by the expression of kappa or lambda light chains, underscoring its significance in B cell function and immune regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Effects of the expansion of bacterial colonies into the intervillous spaces on the localization of several lymphocyte lineages in the rat ileum.

The Journal of veterinary medical science

Yuasa H,Mantani Y,Miyamoto K,Nishida M,Arai M,Tsuruta H,Yokoyama T,Hoshi N,Kitagawa H

MA5-24372 was used in Immunohistochemistry to study the effect of bacterial colonies expanded into the intervillous spaces on the localisation of several lymphocyte lineages.

Tue Apr 16 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: B-lymphocyte antigen CD19; CD19 antigen; Leu-12

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Entrez Gene ID: (Rat) 365367