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          • Primary Antibodies ›
          • CD142 Antibodies
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          Invitrogen

          CD142 Recombinant Rabbit Monoclonal Antibody (34)

          View all (19) CD142 antibodies

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          Datasheet
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          Cite CD142 Recombinant Rabbit Monoclonal Antibody (34)

          • Antibody Testing Data (1)
          CD142 Antibody in Western Blot (WB)
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          CD142 Antibody in Western Blot (WB)
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          CD142 Antibody (MA5-29701) in WB

          Western Blot using CD142 Recombinant Rabbit Monoclonal Antibody (34) (Product # MA5-29701) at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 56 kDa. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CD142 Antibody in Western Blot (WB)

          Product Details

          MA5-29701

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          ELISA (ELISA)

          1:25000-1:50000
          -
          Product Specifications

          Host/Isotype

          Rabbit / IgG

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          34

          Immunogen

          Recombinant Mouse Coagulation Factor III/Tissue Factor/CD142/F3 Protein (Met1-Glu251)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          Lot-Specific

          Storage conditions

          Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

          Shipping conditions

          Wet ice

          RRID

          AB_2785525

          Product Specific Information

          This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

          Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

          This antibody has specificity for Mouse Coagulation Factor III/Tissue Factor/CD142/F3.

          Target Information

          This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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