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Application Notes: Western blot - A reduced sample treatment and SDS-PAGE was used. The band size is ~8 kDa . Positive control: Activated human serum or purified human C3a desArg protein; Negative control: For Western blot, any irrelevant protein can be used.
C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of two chains (110 kDa and 75 kDa) held together by disulfide bonds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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