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Rockland
Brn3b Polyclonal Antibody
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FIGURE: 1 / 3
Brn3b Antibody (600-101-MJ0) in ICC/IF
Immunofluorescence of Goat Anti-Brn3b Antibody. Cell Line: Post-natal rat pup (PND1) heterogeneous brain cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Brn3b at 5µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG DyLight™488 (p/n 605-741-125) at 15µg/mL for 1 hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Brn3b + secondary. (C). Merge A+B. (D). secondary only. Expected localization: Nuclear, nuclear speckle.
Product Details
600-101-MJ0
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Brn3b antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of mouse Pou4f2 conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Form
Liquid
Concentration
1.05 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against mouse Brn3b. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to rat and 91.7% to human.
Target Information
POU4F2 is a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. A class IV POU-domain protein, POU4F2 is found in human retina exclusively within a subpopulation of ganglion cells where it may play a role in determining or maintaining the identities of a small subset of visual system neurons. POU4F2 is a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. A class IV POU-domain protein, POU4F2 is found in human retina exclusively within a subpopulation of ganglion cells where it may play a role in determining or maintaining the identities of a small subset of visual system neurons.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Brain-3B; Brain-specific homeobox/POU domain protein 3B; Brn-3.2; POU domain, class 4, transcription factor 2; POU domain, class 4, transcription factor, related sequence 1
Gene Aliases: Brn-3.2; Brn-3b; Brn3b; mBrn3-3R; Pou4f-rs1; Pou4f2
UniProt ID: (Rat) G3V7L5, (Mouse) Q63934
Entrez Gene ID: (Rat) 171355, (Mouse) 18997
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
molecular_function
chromatin binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
MAPK cascade
transcription, DNA-templated
spermatogenesis
axonogenesis
axon guidance
sensory perception of sound
neuron differentiation
intracellular estrogen receptor signaling pathway
retinal ganglion cell axon guidance
positive regulation of cell differentiation
positive regulation of transcription from RNA polymerase II promoter
axon extension
neuromuscular process controlling balance
retina development in camera-type eye
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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