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Invitrogen
Baculovirus Envelope gp64 Monoclonal Antibody (AcV1), PE, eBioscience™
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FIGURE: 1 / 1
Baculovirus Envelope gp64 Antibody (12-6991-82) in Flow
Insect cells were infected with a GFP-expressing baculovirus and then harvested and stained after 72 hours. Staining with 0.125 µg of Anti-Baculovirus Envelope gp64 Protein PE is presented as a histogram (left) and versus GFP (right). Total viable cells were used for analysis.
Product Details
12-6991-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Published species
Virus
Host/Isotype
Mouse
/ IgG2a
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
AcV1
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1633369
Product Specific Information
Description: The AcV1 monoclonal antibody reacts with the gp64 envelope protein of the baculovirus Autographa californica (AcMNPV). The gp64 envelope protein is essential for virus infectivity and is expressed on the surface of baculovirus-infected cells within six hours of infection. The AcV1 antibody can be used in flow cytometry-based viral titration experiments.
Applications Reported: This AcV1 antibody has been reported for use in flow cytometric analysis. This antibody can be used in identifying virally-infected insect cells and determining viral titers.
Applications Tested: This AcV1 antibody has been tested by flow cytometric analysis of baculovirus-infected insect cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Baculoviruses are known to infect invertebrates, with over 600 species having been described. Immature (larval) forms of moth species are the most common hosts, but these viruses have also been found infecting sawflies, mosquitoes, and shrimp. Although baculoviruses are capable of entering mammalian cells in culture, they are not known to be capable of replication in mammalian or other vertebrate animal cells. The gp64 envelope protein is essential for virus infectivity and is expressed on the surface of baculovirus-infected cells within six hours of infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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