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Invitrogen
BHLHE40 Polyclonal Antibody
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FIGURE: 1 / 3
BHLHE40 Antibody (PA5-22043) in ICC/IF
Immunofluorescence analysis of BHLHE40 was performed using 70% confluent log phase MIA PaCa-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with BHLHE40 Polyclonal Antibody (Product # PA5-22043, 1:100) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790, ... View More
Product Details
PA5-22043
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to a region within amino acids 1 and 81 of SHARP2 (Uniprot ID#O14503)
Conjugate
Form
Liquid
Concentration
0.19 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11155604
Product Specific Information
Recommended positive controls: 293T.
Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Differentiated embryo-chondrocyte expressed gene 1 (DEC1) is an important protein involved in embryonic cell differentiation, proliferation, and apoptosis. It has also recently been shown to be regulated by hypoxia. The loss of DEC1 expression may be an early event in the development of lung cancer, while DEC1 may be up-regulated by hypoxia in other cancers and in more extreme hypoxia it may have a role in cell death.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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