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Rockland
B-Phycoerythrin Polyclonal Antibody
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B-Phycoerythrin Antibody (200-401-099) in WB
Rockland Rabbit anti B-Phycoerythrin antibody (200-4199 lot 25411) was used to detect B-Phycoerythrin under reducing (R) conditions. Reduced samples of purified Phycoerythrin contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of anti B-Phycoerythrin antibody (200-4199 lot 25411 ON 4 C) and detection was using Dylight 649 conjugated Donkey anti rabbit (611-743-127 lot 20831 1:10K 1.5 hr RT in MB-070) and imaged on... View More
Product Details
200-401-099
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phycoerythrin [Porphyridium creuentum]
Conjugate
Form
Liquid
Concentration
1.1 mg/mL
Purification
Ion-exchange chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Phycoerythrin [Porphyridium creuentum].
Target Information
Phycoerythrin B (PEB) is a type of phycobiliprotein found in certain algae, including red algae and cyanobacteria. It is a fluorescent protein that functions as an accessory pigment in the light-harvesting complexes of photosynthetic organisms. Phycoerythrin B absorbs light primarily in the green-yellow range (approximately 480-580 nm) and emits red fluorescence around 580-700 nm. This pigment is highly efficient in capturing light energy and transferring it to chlorophylls during photosynthesis, thereby enhancing the efficiency of the photosynthetic process, especially underwater where light penetration is limited and green light is more prevalent. PEB's unique optical properties make it a valuable tool in biotechnology applications, such as fluorescent labeling and flow cytometry. The protein is composed of alpha and beta subunits that form hexameric structures, each subunit non-covalently binding linear tetrapyrrole chromophores called phycobilins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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