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Rockland
B-Phycoerythrin Polyclonal Antibody
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FIGURE: 1 / 1
B-Phycoerythrin Antibody (100-4199) in WB
Western Blot of Rabbit anti-B-Phycoerythrin antibody. Lane 1: B-Phycoerythrin reduced. Lane 2: B-Phycoerythrin reduced. Lane 3: none. Load: ~1µg per lane. Primary antibody: ß-Phycoerythrin antibody at 1:1000 for overnight at 4°C. Secondary antibody: Dylight 649 conjugated Donkey anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: 18.5 kDa, ~25 kDa for Gli-2. Other band(s): none.
Product Details
100-4199
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
B-Phycoerythrin [Porphyridium cruentum]
Conjugate
Form
Lyophilized
Concentration
75 mg/mL
Storage buffer
0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified B-Phycoerythrin [Porphyridium cruentum]. Cross reactivity against B-Phycoerythrin from other sources is unknown.
Target Information
Phycoerythrin B (PEB) is a type of phycobiliprotein found in certain algae, including red algae and cyanobacteria. It is a fluorescent protein that functions as an accessory pigment in the light-harvesting complexes of photosynthetic organisms. Phycoerythrin B absorbs light primarily in the green-yellow range (approximately 480-580 nm) and emits red fluorescence around 580-700 nm. This pigment is highly efficient in capturing light energy and transferring it to chlorophylls during photosynthesis, thereby enhancing the efficiency of the photosynthetic process, especially underwater where light penetration is limited and green light is more prevalent. PEB's unique optical properties make it a valuable tool in biotechnology applications, such as fluorescent labeling and flow cytometry. The protein is composed of alpha and beta subunits that form hexameric structures, each subunit non-covalently binding linear tetrapyrrole chromophores called phycobilins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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