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Bioss
AmCyan-Tag Monoclonal Antibody (5F9)
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FIGURE: 1 / 1
AmCyan-Tag Antibody (BSM-33184M) in WB
Lane 1:AmCyan-Tagged fusion protein (full length) Overexpression E.coli lysates (Cat#: bs-33184P);Lane 2: Control (-) E.coli lysates probed with AmCyan-Tag Monoclonal Antibody, Unconjugated (bsm-33184M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
Product Details
BSM-33184M
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
5F9
Immunogen
Recombinant AmCyan
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Target Information
AmCyan1 is a human codon-optimized variant of the wild-type Anemonia majano cyan fluorescent protein (AmCyan) that exhibits enhanced emission characteristics (excitation and emission maxima: 458nm and 489nm, respectively). DD-AmCyan1 is a modified version of AmCyan1 that is tagged on its N-terminus with the ProteoTuner destabilization domain (DD). The presence of this destabilization domain causes rapid, proteasomal degradation of the fluorescent fusion protein; however, when the membrane permeant ligand Shield1 is added to the medium, it binds to the destabilization domain and protects the fusion protein from degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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