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Rockland
ASPP1 Polyclonal Antibody
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FIGURE: 1 / 1
ASPP1 Antibody (600-401-A18) in WB
Western blot using Rocklands affinity purified anti-ASPP1 to detect over-expressed ASPP1 in MCF-7 cells (lane 2, arrowhead). Lane 1 is a non-transfected control. Lane 3 is MCF-7 cells over-expressing ASPP2. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1,000 dilution. The identity of the lower MW band at approximately 50kDa is unknown. Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells. Personal Com... View More
Product Details
600-401-A18
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human ASPP1.
Conjugate
Form
Liquid
Concentration
0.33 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Directed against human ASPP1. Minimal reactivity occurs against ASPP2. A BLAST analysis was used to suggest cross-reactivity with ASPP1 from mouse based on a 92% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Target Information
This gene encodes a member of the ASPP family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. ASPP proteins are required for the induction of apoptosis by p53-family proteins. They promote DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Apoptosis-stimulating of p53 protein 1; apoptosis-stimulating protein of p53, 1; Protein phosphatase 1 regulatory subunit 13B; protein phosphatase 1, regulatory (inhibitor) subunit 13B; protein phosphatase 1, regulatory subunit 13B
Gene Aliases: ASPP1; KIAA0771; p53BP2-like; p85; PPP1R13B
UniProt ID: (Human) Q96KQ4
Entrez Gene ID: (Human) 23368
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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