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Invitrogen
AMPK alpha-2 Polyclonal Antibody
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FIGURE: 1 / 3
AMPK alpha-2 Antibody (PA5-29679) in WB
Cancer & metabolism 2021 -
Fig. 4 SIRT6 enhances OXPHOS and energy status in MCF7 cells. a Western blot analysis of PDH expression in MCF7 cells transduced with human WT or catalytically inactive (H133Y) SIRT6 or with a control vector (VECTOR). PDH expression was quantified following normalization to GAPDH. b- g Activity of mitochondrial complexes ( b - d) , oxygen consumption ( e ), activity of F o -F 1 ATP synthase ( f ), and energ... View More
Product Details
PA5-29679
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 202 and 457 of Human AMPK alpha 2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547154
Product Specific Information
PA5-29679 targets AMPK alpha 2 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-29679 immunogen is recombinant fragment corresponding to a region within amino acids 202 and 457 of Human AMPK alpha 2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
Target Information
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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