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Invitrogen
14-3-3 gamma Polyclonal Antibody
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FIGURE: 1 / 1
14-3-3 gamma Antibody (PA1-86556) in WB
Western blot analysis of 14-3-3 isoforms in HEK293 cell extracts using a 14-3-3 gamma polyclonal antibody (Product # PA1-86556) (lane 1), a zeta 14-3-3 (lane 2), a C-epsilon-14-3-3 (lane 3), a N-epsilon-14-3-3 (Lane 4), a eta 14-3-3 (Lane 5). The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3.
Product Details
PA1-86556
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide of acetylated N-terminal amino acids 1-10 of sheep. Peptide sequence: Ac. V D R E Q L V Q K A C of sheep 14-3-3 gamma.
Conjugate
Form
Liquid
Storage conditions
4°C or -20°C if preferred
Shipping conditions
Wet ice
RRID
AB_2217928
Product Specific Information
Predicted to react with all mammals based on sequence homology.
Heat-mediated antigen retrieval is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is normal brain tissue. In Western blot, this antibody detects a band at ~30kDa in HEK293 cell lysate.
Target Information
14-3-3 gamma encodes a product that belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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