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Invitrogen
Human SLC9A5 (aa 544-645) Control Fragment Recombinant Protein
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Product Details
RP-98638
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
GHVLSSTGLTLPSMPSRNSVAETSVTNLLRESGSGACLDLQVIDTVRSGRDREDAVMHHLLCGGLYKPRRRYKASCSRHFISEDAQERQDKEVFQQNMKRRL
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (98%), Rat (98%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-59518. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Na+/H+ exchangers-1-6 (Na+/H+ antiporters, NHE-1-6) are integral membrane proteins that are expressed in most mammalian tissues, where they regulate intracellular pH and cell volume. NHEs mediate the secondary active extrusion of hydrogen (H+) ions out of cells in exchange for extracellular sodium (Na+). Excluding NHE-1, which is ubiquitously expressed, NHE isoforms 2-6 have distinct tissue- and cell type-dependent expression and inhibitiory characteristics by amiloride analogs. Human NHE-5 transcript (solute carrier family 9, isoform-5, SLC9A5) is an 896 amino acid protein that is expressed in brain, testis, spleen and skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Na(+)/H(+) exchanger 5; NHE-5; Sodium/hydrogen exchanger 5; solute carrier family 9 (sodium/hydrogen exchanger), isoform 5; solute carrier family 9 (sodium/hydrogen exchanger), member 5; Solute carrier family 9 member 5; solute carrier family 9, subfamily A (NHE5, cation proton antiporter 5), member 5
Gene Aliases: NHE5; SLC9A5
UniProt ID: (Human) Q14940
Entrez Gene ID: (Human) 6553
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