Invitrogen

Human PRAP1 Control Fragment Recombinant Protein

Product Details

RP-97157

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

PILPGTKAWMETEDTLGRVLSPEPDHDSLYHPPPEEDQGEERPRLWVMPNHQVLLGPEEDQDHIYH

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

1M urea/PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (43%), Rat (43%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-58508. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

Proline rich acidic protein 1 (PRAP1) is also named as PRO1195 or UPA. It may play an important role in maintaining normal growth homeostasis in epithelial cells. Mitotic arrest deficient 1 (MAD1) is a key component in mitotic checkpoint signaling. Recent study identified PRAP1 as a novel MAD1 binding partner using yeast-two hybrid screening. PRAP1 has four isoforms with MW 16-20 kDa and 10 kDa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Epididymis tissue protein Li 178; Proline-rich acidic protein 1; Uterine-specific proline-rich acidic protein