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Invitrogen
Human PPAPDC1B (aa 34-55) Control Fragment Recombinant Protein
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Product Details
RP-106661
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
QRLIQPEEMWLYRNPYVEAEYF
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (91%), Rat (91%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-65973. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Phosphatidate phosphatase (PAP) plays important role in lipid-signaling metabolism in eukaryotic cells. Two distinct types of PAP (PAP1 and PAP2) activity have been distinguished by their subcellular localization and differential sensitivity to N-ethylmaleimide (NEM) and Mg2+. A yeast diacylglycerol pyrophosphate (DGPP) phosphatase (DPP1) and mammalian DGPP phosphatase (PAP2) have been identified as Mg2+-independent and NEM-insensitive membrane-associated. PPAPDC1A (also known as DPPL2) and PPAPDC1B (DPPL1) form a novel type of Mg2+-independent and NEM-sensitive mammalian phosphatidate phosphatase showing broad substrate specificity. Knockdown experiments indicated that this protein is involved with multiple cell signaling pathways, including the JAK-Stat3, MAP kinase, and PKC pathways. PPAPDC1B may also potentiate the estrogen receptor pathway by down-regulating DUSP22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: diacylglycerol pyrophosphate like 1; diacylglycerol pyrophosphate phosphatase-like 1; phosphatidate phosphatase PPAPDC1B; phosphatidic acid phosphatase type 2 domain containing 1B; Phosphatidic acid phosphatase type 2 domain-containing protein 1B; Phospholipid phosphatase 5; testicular secretory protein Li 38
Gene Aliases: DPPL1; HTPAP; PLPP5; PPAPDC1B
UniProt ID: (Human) Q8NEB5
Entrez Gene ID: (Human) 84513
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