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Invitrogen
Human POU6F1 (aa 108-147) Control Fragment Recombinant Protein
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Product Details
RP-106284
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
KPSASAPIPITCSETPTVSQLVSKPHTPSLDEDGINLEEI
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (95%), Rat (95%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-65617. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Brn-5 (Brain-5), also known as POU6F1, MPOU or TCFB1, is a widely expressed protein but during embryogenesis is exclusively found in the developing brain and spinal cord. As is characteristic of Brn family members, Brn-5 contains two DNA-binding domains, namely the POU-specific domain and the POU homeodomain, which each contain an HTH (helix-turn-helix) motif. Brn-5 binds to CRH (corticotrophin-releasing hormone) elements with high affinity and is capable of both enhancing Prolactin gene expression and activating Pit-1 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Brain-5; Brain-specific homeobox/POU domain protein 5; brn-5; mPOU homeobox protein; POU domain, class 6, transcription factor 1
Gene Aliases: BRN5; MPOU; POU6F1; TCFB1
UniProt ID: (Human) Q14863
Entrez Gene ID: (Human) 5463
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