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Invitrogen
Human POU5F1B (aa 51-101) Control Fragment Recombinant Protein
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Product Details
RP-105098
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
VGPGSEVWGIPPCPPPYELCGGMAYCGPQVGVGLVPQGGLETSQPESEAGV
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (76%), Rat (76%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-66155. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
This intronless gene was thought to be a transcribed pseudogene of POU class 5 homeobox 1, however, it has been reported that this gene can encode a functional protein. The encoded protein is nearly the same length as and highly similar to the POU class 5 homeobox 1 transcription factor, has been shown to be a weak transcriptional activator and may play a role in carcinogenesis and eye development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Oct4-pg1; octamer binding protein 3_like sequence; Octamer-binding protein 3-like; Octamer-binding transcription factor 3-like; POU 5 domain protein; POU class 5 homeobox 1 pseudogene 1; POU domain transcription factor Oct-4; POU domain transcription factor OCT4-pg1; POU domain, class 5, transcription factor 1 pseudogene 1; POU domain, class 5, transcription factor 1B
Gene Aliases: OCT4-PG1; OCT4PG1; OTF3C; OTF3P1; POU5F1B; POU5F1P1; POU5F1P4; POU5FLC20; POU5FLC8
UniProt ID: (Human) Q06416
Entrez Gene ID: (Human) 5462
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