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Invitrogen
Human PIGF (aa 132-153) Control Fragment Recombinant Protein
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Product Details
RP-101375
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
GPNLKAWLRVFSRNGVTSIWEN
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (100%), Rat (100%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-62396. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Phosphatidylinositol-glycans(PIGs) aremulti-passtransmembrane proteins that localize to the endoplasmicreticulum. PIGs exhibitvariousfunctions but all are crucial for the biosynthesis of the glycosylphosphatidylinositol (GPI) anchor. Some PIG proteins are components of the GPI transamidase complex and play a role in the recognition of either the GPI attachmentsignal or the lipid portion of GPI. Other PIGs belong to the glycosyltransferase complex and function in the transfer of N-acetylglucosamine (GlcNAc) to phosphatidyli nositol (PI). A variety of other PIGs play distinct rolesin GPIsynthesis. PIG-F functions as an auxiliary subunit of ethanolamine phosphate (EtNP) transferases. It associates with PIG-O and isrequired for its expression and stability. Together these two PIGs function as an EtNP transferase and catalyze the transfer of EtNP to the third mannose (Man-3) of GPI. Amutation in the gene encoding PIG-F may result in a block of EtNP addition to Man-3 and lead to an absence of GPI-anchored proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GPI ethanolamine phosphate transferase 2, stabilizing subunit; GPI ethanolamine phosphate transferase 3, stabilizing subunit; GPI ethanolamine phosphate transferase, stabilizing subunit; GPI11 homolog; phosphatidylinositol glycan anchor biosynthesis, class F; Phosphatidylinositol-glycan biosynthesis class F protein; PIG-F
Gene Aliases: PIGF
UniProt ID: (Human) Q07326
Entrez Gene ID: (Human) 5281
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