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Invitrogen
Human PHYHD1 (aa 19-138) Control Fragment Recombinant Protein
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Product Details
RP-89700
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
VLEGFLSAEECVAMQQRIGEIVAEMDVPLHCRTEFSTQEEEQLRAQGSTDYFLSSGDKIRFFFEKGVFDEKGNFLVPPEKSINKIGHALHAHDPVFKSITHSFKVQTLARSLGLQMPVVV
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (90%), Rat (90%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-110772. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
PHYHD1 (phytanoyl-CoA dioxygenase domain containing 1) is a 291 amino acid protein belonging to the PHYH family and the PHYHD1 subfamily. Encoded by a gene that maps to human chromosome 9q34.11, PHYHD1 exists as three alternatively spliced isoforms and likely functions as an alpha-ketoglutatare-dependent dioxygenase. PHYHD1 participates in metal ion binding and oxidoreductase activity, thereby acting on single donors with incorporation of two atoms of oxygen. Related to PHYH (phytanoyl-CoA 2-hydroxylase), an Fe(II) and 2-oxoglutarate (2OG)-dependent oxygenase that catalyzes the initial alpha-oxidation step in the degradation of phytenic acid in peroxisomes, PHYHD1 similarly exhibits additional homologues in a wide range of metazoans and bacteria. PHYHD1 also may play a role in DNA methylation in early postnatal liver development and mammalian differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Phytanoyl-CoA dioxygenase domain-containing protein 1; Protein PHYHD1
Gene Aliases: PHYHD1
UniProt ID: (Human) Q5SRE7
Entrez Gene ID: (Human) 254295
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