Gibco

Human Osteopontin Recombinant Protein, PeproTech®

Product Details

120-35-50UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 8 publications 8 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Human, Mouse

Expression System

HEK293 cells

Amino acid sequence

IPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLA PQNAVSSEET NDFKQETLPS KSNESHDHMD DMDDEDDDDH VDSQDSIDSN DSDDVDDTDD SHQSDESHHS DESDELVTDF PTDLPATEVF TPVVPTVDTY DGRGDSVVYG LRSKSKKFRR PDIQYPDATD EDITSHMESE ELNGAYKAIP VAQDLNAPSD WDSRGKDSYE TSQLDDQSAE THSHKQSRLY KRKANDESNE HSDVIDSQEL SKVSREFHSH EFHSHEDMLV VDPKSKEEDK HLKFRISHEL DSASSEVN

Molecular weight

33.7 kDa

Class

Recombinant

Type

Protein

Purity

≥ 97% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to enhance cell adhesion of murine B16-F1 cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant human Osteopontin is a 298 amino acid protein, which, due to glycosylation, migrates at an apparent molecular weight of 60.0-65.0 kDa by SDS-PAGE analysis under reducing conditions.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Obesity-induced senescent macrophages activate a fibrotic transcriptional program in adipocyte progenitors.

Life science alliance

Rabhi N,Desevin K,Belkina AC,Tilston-Lunel A,Varelas X,Layne MD,Farmer SR

120-35 was used in Cell Culture to reveal that obesity promotes macrophage senescence and provides a conceptual framework for the discovery of rational therapeutic targets for metabolic and inflammatory disease associated with obesity.

Sun May 01 00:00:00 UTC 2022

ADAM8 affects glioblastoma progression by regulating osteopontin-mediated angiogenesis.

Biological chemistry

Li Y,Guo S,Zhao K,Conrad C,Driescher C,Rothbart V,Schlomann U,Guerreiro H,Bopp MH,König A,Carl B,Pagenstecher A,Nimsky C,Bartsch JW

120-35 was used in In vitro experiments to conclude that ADAM8 could be a tractable target to modulate angiogenesis in GBM with minor side-effects.

Wed Jan 27 00:00:00 UTC 2021

COVID-19 and RA share an SPP1 myeloid pathway that drives PD-L1+ neutrophils and CD14+ monocytes.

JCI insight

MacDonald L,Alivernini S,Tolusso B,Elmesmari A,Somma D,Perniola S,Paglionico A,Petricca L,Bosello SL,Carfì A,Sali M,Stigliano E,Cingolani A,Murri R,Arena V,Fantoni M,Antonelli M,Landi F,Franceschi F,Sanguinetti M,McInnes IB,McSharry C,Gasbarrini A,Otto TD,Kurowska-Stolarska M,Gremese E

Fri Jun 18 00:00:00 UTC 2021

Single-Cell Analyses Inform Mechanisms of Myeloid-Targeted Therapies in Colon Cancer.

Cell

Zhang L,Li Z,Skrzypczynska KM,Fang Q,Zhang W,O'Brien SA,He Y,Wang L,Zhang Q,Kim A,Gao R,Orf J,Wang T,Sawant D,Kang J,Bhatt D,Lu D,Li CM,Rapaport AS,Perez K,Ye Y,Wang S,Hu X,Ren X,Ouyang W,Shen Z,Egen JG,Zhang Z,Yu X

Thu Apr 16 00:00:00 UTC 2020

A low MW inhibitor of CD44 dimerization for the treatment of glioblastoma.

British journal of pharmacology

Wang C,Wang Z,Chen C,Fu X,Wang J,Fei X,Yan X,Xu R

120-35 was used in In vitro experiments to predict verbascoside as a candidate CD44 inhibitor using the systemsDock software and demonstrate that verbascoside suppresses growth of glioblastoma cells by inhibiting CD44 dimerization.

Wed Jul 01 00:00:00 UTC 2020

Squalene synthase promotes the invasion of lung cancer cells via the osteopontin/ERK pathway.

Oncogenesis

Yang YF,Chang YC,Jan YH,Yang CJ,Huang MS,Hsiao M

Sat Aug 29 00:00:00 UTC 2020

Growth Factor-like Gene Regulation Is Separable from Survival and Maturation in Antibody-Secreting Cells.

Journal of immunology (Baltimore, Md. : 1950)

Stephenson S,Care MA,Fan I,Zougman A,Westhead DR,Doody GM,Tooze RM

Fri Feb 15 00:00:00 UTC 2019

A Sensitized IGF1 Treatment Restores Corticospinal Axon-Dependent Functions.

Neuron

Liu Y,Wang X,Li W,Zhang Q,Li Y,Zhang Z,Zhu J,Chen B,Williams PR,Zhang Y,Yu B,Gu X,He Z

Wed Aug 16 00:00:00 UTC 2017

Oncostatin M promotes cancer cell plasticity through cooperative STAT3-SMAD3 signaling.

Oncogene

Junk DJ,Bryson BL,Smigiel JM,Parameswaran N,Bartel CA,Jackson MW

Thu Jul 13 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: Bone sialoprotein 1; Bone sialoprotein 1 (BSPI/BSP1); CALPHA1 fusion; Early T lymphocyte activation 1 (ETA1); early T-lymphocyte activation 1; HGNC:11255; immunoglobulin alpha 1 heavy chain constant region fusion protein; MGC110940; Nephropontin; Osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; Secreted phosphoprotein 1; secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1); secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1 (SPP1); secreted phosphoprotein 1 variant 6; SPP-1; SPP1/CALPHA1 fusion; urinar; Urinary stone protein; Uropontin

Human Osteopontin Recombinant Protein, PeproTech®

Product Details

120-35-50UG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (MISC)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

We're here to help

Your items have has been added!