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Invitrogen
Human MMP-9 Recombinant Protein
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Product Details
RP-75654
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
Amino acids Asp113- Glu450 containing an N-terminal His-tag
Tag
His-tag
Molecular weight
42.03 kDa
Class
Recombinant
Type
Protein
Purity
>95%, by SDS-PAGE under reducing conditions
Conjugate
Form
Liquid
Concentration
0.27 mg/mL
Purification
purified
Storage buffer
20mM tris HCl, pH 8.0, with 50% glycerol
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
This product contains purified recombinant human MMP-9 protein with an N-terminal hexahistidine tag. The recombinant protein is a fragment corresponding to the catalytic domain of the protein (residues 113-450).
This product may be used as a positive control in many immunological assays.
Target Information
MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; Gelatinase-B; GELB; macrophage gelatinase; matrix metallo protease; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); Matrix metalloproteinase-9; Matrix metalloproteinase9; MMP; MMP-9; MMPs; type V collagenase
Gene Aliases: CLG4B; GELB; MANDP2; MMP-9; MMP9
UniProt ID: (Human) P14780
Entrez Gene ID: (Human) 4318
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