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          • Proteins & Peptides ›
          • MIF Proteins
          The PeproTech website has now moved to Thermofisher.com. All orders for PeproTech products must be placed on thermofisher.com or offline via phone, fax, email, or distributor partner. For any questions, please contact PeproTech.CustomerService@thermofisher.com

          Gibco

          Human MIF Recombinant Protein, PeproTech®

          9 References
          View all (3) MIF proteins

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          Cite Human MIF Recombinant Protein, PeproTech®

          • Testing Data (1)
          Human MIF Protein in Functional Assay (Functional)
          Group 53 Created with Sketch.
          Human MIF Protein in Functional Assay (Functional)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Human MIF Protein (300-69-25UG) in Functional

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          Human MIF Protein in Functional Assay (Functional)

          Product Details

          300-69-25UG

          Applications
          Tested Dilution
          Publications

          Functional Assay (Functional)

          Assay-dependent
          -

          In vitro Assay (IV)

          -
          View 5 publications 5 publications

          Miscellaneous PubMed (MISC)

          -
          View 4 publications 4 publications
          Product Specifications

          Species

          Human

          Published species

          Human, Mouse

          Expression System

          Insect cells

          Amino acid sequence

          HHHHHHHHAM PMFIVNTNVP RASVPDGFLS ELTQQLAQAT GKPPQYIAVH VVPDQLMAFG GSSEPCALCS LHSIGKIGGA QNRSYSKLLC GLLAERLRIS PDRVYINYYD MNAANVGWNN STFA

          Tag

          His-tag

          Molecular weight

          15 kDa

          Class

          Recombinant

          Type

          Protein

          Purity

          ≥ 98% by SDS-PAGE gel and HPLC analyses.

          Endotoxin concentration

          <1 EU/µg

          Activity

          Determined by its ability to inhibit monocyte migration.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Purification

          purified

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient

          Product Specific Information

          Insect cell-derived Recombinant Human MIF is a 15 kDa protein containing 124 amino acid residues, including an N-terminal His-tag.

          This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

          Target Information

          MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: GIF; Glycosylation-inhibiting factor; HGNC:7097; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; MGC107654; MIF; Phenylpyruvate tautomerase

          View more View less

          Gene Aliases: GIF; GLIF; MIF; MMIF

          View more View less

          UniProt ID: (Human) P14174

          View more View less

          Entrez Gene ID: (Human) 4282

          View more View less

          It has to be done as per old AB suggested Products section.
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