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Invitrogen
Human IL-7 Recombinant Protein
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Product Details
RIL75
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
Human IL-7 recombinant protein contains 153 amino acids
Molecular weight
17.4 kDa
Class
Recombinant
Type
Protein
Purity
>98% by SDS-PAGE and HPLC
Activity
ED50 ≤ 0.5 ng/mL; determined by the dose-dependent proliferation of mouse 2E8 cells.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Use: Human IL-7 exerts activity in the range of 0.1 to 10 ng/mL for most in vitro applications.
Reconstitution and Dilution: Reconstitute in distilled water to a concentration of 100 µg/mL. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V).
Lyophilized product is stable until expiration if stored desiccated below 0°C. Cytokines diluted as indicated above should be divided into aliquots and stored at -70°C. Recombinant proteins should be stored at concentrations >500 ng/mL to reduce loss of activity.
Target Information
Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD127; CD127 antigen; CDw127; H-IL-7; IL-7; IL-7 receptor subunit alpha; IL-7R subunit alpha; ILN; Interleukin; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; Interleukin-7; Interleukin-7 receptor subunit alpha; Interleukin7; M-IL-7
Gene Aliases: CD127; CDW127; IL-7; IL-7R-alpha; IL7; IL7R; IL7RA; ILRA
UniProt ID: (Human) P13232, (Human) P16871
Entrez Gene ID: (Human) 3574, (Human) 3575
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