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Invitrogen
Human CD40 (TNFRSF5) His-tag Recombinant Protein
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Product Details
A42565
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human CD40, amino acids Met1-Arg193 (Accession # NP_001241.1) with a C-terminal His-tag
Tag
His-tag
Molecular weight
20.7 kDa
Class
Recombinant
Type
Protein
Purity
≥95 % as determined by SDS-PAGE. ≥95 % as determined by SEC-HPLC.
Endotoxin concentration
<1.0 EU/µg
Activity
1.Immobilized Human CD40 (TNFRSF5) His-tag Recombinant Protein at 2 µg/mL (100 µL/well) can bind Human CD40 Ligand (ECD, hFc & AVI Tag), Biotinylated, the EC50 is 6-20 ng/mL.
2.Loaded Human CD40 (TNFRSF5) His-tag Recombinant Protein on His1K Biosensor, can bind Recombinant Human CD40 Ligand Protein, hFc Tag with an affinity constant of 5.88 nM as determined in BLI assay (Sartorius Octet RH16) (Routinely tested).
2.Loaded Human CD40 (TNFRSF5) His-tag Recombinant Protein on His1K Biosensor, can bind Recombinant Human CD40 Ligand Protein, hFc Tag with an affinity constant of 5.88 nM as determined in BLI assay (Sartorius Octet RH16) (Routinely tested).
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
CD40 is a single-chain glycoprotein and a member of the tumor necrosis factor receptor (TNFR) family, exhibiting significant homology to the Hodgkin's disease-associated antigen, CD30. It is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells, as well as some epithelial cells, carcinomas, and lymphoid dendritic cells. Notably, CD40 is present on all B cells except plasma cells. CD40 plays a crucial role in regulating B cell development and maturation, inducing immunoglobulin isotype-switching, and protecting B cells from surface Ig-induced apoptosis when combined with other signals such as IL-4. It promotes proliferation and is essential for T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. The interaction between CD40 and its ligand CD154 (gp39) on T cells is vital for T-B cell crosstalk, costimulation, and immune regulation. Adaptor protein TNFR2 interacts with CD40, mediating signal transduction, while the AT-hook transcription factor AKNA is reported to regulate CD40 expression, which may be important for homotypic cell interactions. Additionally, the interaction between CD40 and its ligand is necessary for amyloid-beta-induced microglial activation, suggesting a role in the early pathogenesis of Alzheimer's disease. Two alternatively spliced transcript variants encoding distinct isoforms of CD40 have been identified. Diseases associated with CD40 dysfunction include Type 3 Hyper-IgM immunodeficiency and CD40 ligand deficiency, highlighting its importance in immune and inflammatory responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD antigen CD40; CD40; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; CDw40; Immunoglobulin M; ImmunoglobulinM; MGC9013; sCD40; soluble CD40; Tumor necrosis factor receptor superfamily member 5
Gene Aliases: Bp50; CD40; CDW40; p50; TNFRSF5
UniProt ID: (Human) P25942
Entrez Gene ID: (Human) 958
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