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Invitrogen
Human ATP6V0A2 (aa 54-189) Control Fragment Recombinant Protein
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Product Details
RP-91288
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
EVKRCEELERILVYLVQEINRADIPLPEGEASPPAPPLKQVLEMQEQLQKLEVELREVTKNKEKLRKNLLELIEYTHMLRVTKTFVKRNVEFEPTYEEFPSLESDSLLDYSCMQRLGAKLGFVSGLINQGKVEAFE
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (94%), Rat (94%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-60665. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
The multisubunit vacuolar-type proton pump (H(+)-ATPase or V-ATPase) is essential for acidification of diverse cellular components, including endosomes, lysosomes, clathrin-coated vesicles, secretory vesicles, and chromaffin granules, and it is found at high density in the plasma membrane of certain specialized cells. H(+)-ATPases are comprised of a peripheral V(1) domain and an integral membrane V(0) domain; ATP6V0A2 is a component of the V(0) domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A2V-ATPase; ATP6 ATP6N1D; ATPase, H+ transporting, lysosomal V0 subunit a2; Lysosomal H(+)-transporting ATPase V0 subunit a 2; lysosomal H(+)-transporting ATPase V0 subunit a2; regeneration and tolerance factor; TJ6; v-ATPase 116 kDa; V-ATPase 116 kDa subunit a 2; v-type proton ATPase 116 kDa subunit a; V-type proton ATPase 116 kDa subunit a 2; vacuolar proton translocating ATPase 116 kDa subunit a; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 2
Gene Aliases: A2; ARCL; ARCL2A; ATP6A2; ATP6N1D; ATP6V0A2; J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS
UniProt ID: (Human) Q9Y487
Entrez Gene ID: (Human) 23545
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