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Reconstitute the lyophilized antiserum by add ing 1 mL sterile distilled water.
The antiSerum does not cross-react with any other component of Swine plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion anti several sera with the following results: Bovine - Cat - Chicken - Dog - Duck - Guinea Pig - Hamster - Horse - Human - Monkey - Mouse - pigeon - Rat - Sheep - Turkey-.
In immunoelectrophoresis use 2 mL or equivalent against 120 mL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 mL in a 3 mm diameter center well and 2 mL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day.
Haptoglobin is an a2-globulin plasma protein synthesized in the liver. It is an acute phase protein which also acts as a scavenger of hemoglobin from hemolyzed red cells. The haptoglobin monomer consists of two a and two beta chains; the a chains bay be hp1Fa, hp1Sa, or hp2a types while the beta chain is constant. Three major phenotypes are recognized: HP1-1, Hp2-1 and Hp-2-2, in which the hp1a chain bay be either the F or S type.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: alpha polypeptide; beta polypeptide; Binding peptide (BP); Haptoglobin; haptoglobin alpha 1S; Haptoglobin alpha chain / alpha(1S) beta; Haptoglobin alpha(2FS) beta; HP2 ALPHA2; HP2-ALPHA-2; HPT; MGC111141; MGC133752; zonulin
Gene Aliases: HP
UniProt ID: (Pig) Q8SPS7
Entrez Gene ID: (Pig) 397061
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