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Bethyl Laboratories
ZBP89 Polyclonal Antibody
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FIGURE: 1 / 1
ZBP89 Antibody (A303-116A) in WB
Detection of human and mouse ZBP89 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ZBP89 antibody (Product # A303-116A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 6 µg/mg lysate. ZBP89 was also immunoprecipitated by rabbit anti-ZBP89 antibody A303-117A, which recognizes a downstream epitope. Detection: Chemiluminescence with ... View More
Product Details
A303-116A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 375 and 425 of Human Zinc Finger DNA-Binding Protein 89.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Bovine and Orangutan
Target Information
ZNF148 is involved in transcriptional regulation. ZNF148 represses the transcription of a number of genes including gastrin, stromelysin and enolase. ZNF148 binds to the G-rich box in the enhancer region of these genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Beta enolase repressor factor 1; CACCC box-binding protein; CLL-associated antigen KW-10; G-rich box-binding protein; Transcription factor BFCOL1; Transcription factor ZBP-89; Zinc finger DNA-binding protein 89; Zinc finger protein 148
Gene Aliases: 2210405J08Rik; AI480666; AW045217; BERF-1; BFCOL1; HT-BETA; pHZ-52; ZBP-89; ZBP89; ZFP148; ZNF148
UniProt ID: (Human) Q9UQR1, (Mouse) Q61624
Entrez Gene ID: (Human) 7707, (Mouse) 22661
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein binding
sequence-specific DNA binding
metal ion binding
transcription regulatory region sequence-specific DNA binding
nucleic acid binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
protein complex assembly
gamete generation
negative regulation of gene expression
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cellular defense response
substantia nigra development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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