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Invitrogen
SARS/SARS-CoV-2 Spike Protein (S1/S2) Polyclonal Antibody
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SARS/SARS-CoV-2 Spike Protein (S1/S2) Antibody (PA5-112049) in ELISA
An ELISA was performed using human SARS-CoV-2 Spike S1 recombinant protein as coating antigen and the SARS-CoV/SARS-CoV-2 Spike Protein Polyclonal Antibody (Product # PA5-112049), as the capture antibody. A Goat anti-Rabbit IgG (HRP) secondary antibody was used at a 1:20,000 dilution. Detection range is from 16 ng/mL to 4000 ng/mL.
Product Details
PA5-112049
Product Specifications
Species Reactivity
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
peptide corresponding to 17 amino acids near the center of SARS-CoV Spike glycoprotein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2866785
Product Specific Information
The immunogen is located within amino acids 550-600 of SARS-CoV Spike.
Predicted reactivity based on immunogen sequence: SARS-CoV2 Spike protein: (identity 65%, homology 88%)
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. SARS-CoV-2 S is 1273 amino acids long and consists of a signal peptide (amino acids 1-13) located at the N-terminus, the S1 subunit (14-685 residues), and the S2 subunit (686-1273 residues); the last two regions are responsible for receptor binding and membrane fusion, respectively. The S-ECD is the extracellular domain of the Spike protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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