Rockland

Phospho-STAT3 (Tyr705) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

600-401-C64

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

ELISA (ELISA)

1:10,000-1:30,000
-

Immunoprecipitation (IP)

1 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified Stat3 antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids surrounding tyrosine 705 of human Stat3.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-STAT3 antibody is an affinity-purified antibody and is directed against the phosphorylated form of human STAT3 protein at the Y705. Anti-Stat3 antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated Stat3 protein is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross reactivity with Stat3 antibodies from human, mouse, rat, and pig based on 100%sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

Target Information

STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Acute-phase response factor; DNA-binding protein APRF; FLJ20882; MGC16063; Signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 (acute-phase response factor); STAT

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Gene Aliases: ADMIO; ADMIO1; APRF; HIES; STAT3

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UniProt ID: (Human) P40763

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Entrez Gene ID: (Human) 6774

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II repressing transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding signal transducer activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding transcription factor binding protein kinase binding protein phosphatase binding chromatin DNA binding CCR5 chemokine receptor binding glucocorticoid receptor binding identical protein binding transcription regulatory region DNA binding protein dimerization activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter temperature homeostasis eye photoreceptor cell differentiation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein import into nucleus movement of cell or subcellular component acute-phase response signal transduction JAK-STAT cascade nervous system development aging cell proliferation negative regulation of cell proliferation positive regulation of gene expression negative regulation of hydrogen peroxide biosynthetic process viral process phosphorylation cytokine-mediated signaling pathway sexual reproduction intracellular receptor signaling pathway response to estradiol cellular response to hormone stimulus leptin-mediated signaling pathway somatic stem cell population maintenance miRNA mediated inhibition of translation regulation of multicellular organism growth response to drug positive regulation of tyrosine phosphorylation of Stat3 protein glucose homeostasis eating behavior negative regulation of apoptotic process cellular response to leptin stimulus response to leptin response to ethanol positive regulation of Notch signaling pathway negative regulation of glycolytic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of mitochondrial membrane permeability astrocyte differentiation regulation of cell cycle radial glial cell differentiation regulation of feeding behavior growth hormone receptor signaling pathway JAK-STAT cascade involved in growth hormone signaling pathway interleukin-6-mediated signaling pathway cellular response to organic cyclic compound energy homeostasis negative regulation of neuron death positive regulation of growth factor dependent skeletal muscle satellite cell proliferation positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of metalloendopeptidase activity positive regulation of gene silencing by miRNA positive regulation of ATP biosynthetic process negative regulation of neuron migration
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