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Invitrogen
PSMB10 Recombinant Rabbit Monoclonal Antibody (23GB2165)
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FIGURE: 1 / 3
PSMB10 Antibody (MA5-53238) in WB
Western Blot analysis of PSMB10. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSMB10 recombinant monoclonal antibody (Product # MA5-53238) with a dilution of 1:5,000, followed by HRP-conjugated goat anti-rabbit secondary antibody with a dilution of 1:20,000. Image was developed using FeQTM ECL Substrate Kit.
Product Details
MA5-53238
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
23GB2165
Immunogen
A synthesized peptide derived from human PSMB10 (50-80AA).
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3247711
Target Information
The 20S proteasome is a protease complex that is responsible for cytosolic protein degradation and generation of peptide ligands for major histocompatibility complex (MHC) class I molecules, either in their final form or in the form of amino-terminally extended precursors. Upon IFN-gamma stimulation of cells, three constitutively expressed subunits of the 20S proteasome are replaced by inducible subunits LMP2 (low-molecular mass polypeptide 2), LMP7, and MECL-1 (multicatalytic endopeptidase complex-like-1, LMP10). LMP2, LMP7, and MECL-1 subunits form immunoproteasomes, which are associated with more efficient class I antigen processing and presentation. Independent assortment of LMP-2, LMP-7, and MECL-1 into different proteasome complexes can lead to 36 unique proteasome subsets, which may mediate differences in the cleavage specificities/cleavage motifs of proteins subject to constitutive- and immuno-proteasomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Low molecular mass protein 10; Macropain subunit MECl-1; MGC1665; Multicatalytic endopeptidase complex subunit MECl-1; OTTHUMP00000174858; prosome Mecl1; proteasome (prosome, macropain) subunit, beta type, 10; proteasome (prosomome, macropain) subunit, beta type 10; proteasome catalytic subunit 2i; Proteasome MECl 1; Proteasome MECl-1; proteasome subunit beta 7i; Proteasome subunit beta type-10; Proteasome subunit beta-2i; proteasome subunit MECL1; PSMB 10
Gene Aliases: beta2i; LMP10; Mecl-1; MECL1; PSMB10
UniProt ID: (Human) P40306, (Rat) Q4KM35, (Mouse) O35955
Entrez Gene ID: (Human) 5699, (Rat) 291983, (Mouse) 19171
cell morphogenesis
T cell proliferation
proteolysis involved in cellular protein catabolic process
proteolysis
proteasomal ubiquitin-independent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
MAPK cascade
protein polyubiquitination
stimulatory C-type lectin receptor signaling pathway
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
regulation of cellular amino acid metabolic process
humoral immune response
viral process
anaphase-promoting complex-dependent catabolic process
tumor necrosis factor-mediated signaling pathway
NIK/NF-kappaB signaling
Fc-epsilon receptor signaling pathway
regulation of mRNA stability
T cell receptor signaling pathway
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
Wnt signaling pathway, planar cell polarity pathway
negative regulation of canonical Wnt signaling pathway
positive regulation of canonical Wnt signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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