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Invitrogen
FGF6 Polyclonal Antibody
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FIGURE: 1 / 1
FGF6 Antibody (PA5-47306) in Neu
Neutralization of FGF6 in NR6R‚3T3 mouse fibroblast cell line. Samples were incubated in FGF6 polyclonal antibody (Product # PA5-47306). Recombinant Human FGF‚6 stimulates proliferation in the NR6R‚3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‚6 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF‚6 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.2-1 µg/mL in the presence of heparin (1 µg/mL).
Product Details
PA5-47306
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human FGF-6 Gly41-Ile208
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609519
Product Specific Information
In Western blots, approximately 50% cross-reactivity with recombinant human (rh) FGF-4 is observed, less than 5% cross-reactivity with rhFGF-9 is observed, and less than 2% cross-reactivity with FGF-acidic, rhFGF-5, and rhFGF-7 is observed. In direct ELISAs, less than 1% cross-reactivity with rh beta -ECGF, recombinant mouse (rm) FGF-8b, and rmFGF-8c is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
FGF-6 is a secreted, heparin-binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-6 is expressed in leukemia cell lines with platelet megakaryocytic differentiation potential. It signals through FGFR 1c, 2c, and 4.4
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FGF; FGF-6; Fibroblast growth factor; Fibroblast growth factor 6; HBGF-6; Heparin secretory-transforming protein 2; Heparin-binding growth factor 6; HST-2; HSTF-2
Gene Aliases: FGF6; HBGF-6; HST2; HSTF2
UniProt ID: (Human) P10767
Entrez Gene ID: (Human) 2251
MAPK cascade
cartilage condensation
angiogenesis
signal transduction
cell-cell signaling
cell proliferation
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
phosphatidylinositol-3-phosphate biosynthetic process
positive regulation of GTPase activity
myoblast differentiation
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
positive regulation of cell division
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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