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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • E-cadherin Antibodies
          The product (cat # {{productObj.productId}}) that you're looking for has been discontinued and replaced with {{productObj.replacementProductId}}. The product (cat # {{productObj.productId}}) that you're looking for has been discontinued. Please contact Technical Support with any questions.

          Invitrogen

          E-cadherin Polyclonal Antibody

          1 Published Figure
          1 Reference
          View all (156) E-cadherin antibodies

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          Datasheet
          Protocols
          Datasheet
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          Cite E-cadherin Polyclonal Antibody

          • Antibody Testing Data (5)
          • Published Figures (1)
          E-cadherin Antibody in Immunocytochemistry (ICC/IF)
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          E-cadherin Antibody in Immunocytochemistry (ICC/IF)
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          FIGURE: 1 / 6

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          E-cadherin Antibody (PA5-80457) in ICC/IF

          Immunofluorescence staining of Human E-CAD in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with E-cadherin Polyclonal Antibody (Product # PA5-80457, 1:200). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cells membrane. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          E-cadherin Antibody in Immunocytochemistry (ICC/IF)
          E-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          E-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          E-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          E-cadherin Antibody in Western Blot (WB)
          E-cadherin Antibody in Immunocytochemistry (ICC/IF)

          Product Details

          PA5-80457

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:500-1:2,000
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:1,000
          View 1 publication 1 publication

          ELISA (ELISA)

          1:5,000-1:10,000
          -
          Product Specifications

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant Human E-Cadherin/CDH1/E-cad/CD324 protein (Met1-Ile707)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Storage conditions

          Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2787771

          Product Specific Information

          This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

          This antibody has specificity for Human E-Cadherin/CDH1/E-cad/CD324.

          Target Information

          E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
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