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Invitrogen
CD40 Recombinant Human Monoclonal Antibody (5D12)
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Product Details
MA5-51892
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cynomolgus monkey,
Human,
Non-human primate
Host/Isotype
Human
/ IgG4, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5D12
Immunogen
Anti-CD40 mAb 5D12, was generated by immunizing mice with sf9 insect cells expressing recombinant human CD40 and selected for the ability to bind EBV-immortalized human B cells. The variable regions from mu5D12 were cloned and used to construct chimeric humanized IgG4 5D12.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3250584
Product Specific Information
ch5D12 binds specifically to recombinant and native human CD40 ectodomain and has been shown to also bind CD40 in cynomolgus and marmoset monkeys. The antibody requires residues within D1 and D1/B2 for binding (D1 domain is crucial for CD40L binding). Binding characteristics are very similar between humanized ch5D12 and mu5D12. CD40 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40 binds to CD40L, and this interaction is involved in B-cell activation and proliferation, antigen-presenting cell (APC) activation, initiation of antigen-specific T-cell responses, immunoglobulin production, activation of effector macrophages isotype switching, homotypic adhesion and rescue from apoptosis.
Target Information
CD40 is a single-chain glycoprotein and a member of the tumor necrosis factor receptor (TNFR) family, exhibiting significant homology to the Hodgkin's disease-associated antigen, CD30. It is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells, as well as some epithelial cells, carcinomas, and lymphoid dendritic cells. Notably, CD40 is present on all B cells except plasma cells. CD40 plays a crucial role in regulating B cell development and maturation, inducing immunoglobulin isotype-switching, and protecting B cells from surface Ig-induced apoptosis when combined with other signals such as IL-4. It promotes proliferation and is essential for T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. The interaction between CD40 and its ligand CD154 (gp39) on T cells is vital for T-B cell crosstalk, costimulation, and immune regulation. Adaptor protein TNFR2 interacts with CD40, mediating signal transduction, while the AT-hook transcription factor AKNA is reported to regulate CD40 expression, which may be important for homotypic cell interactions. Additionally, the interaction between CD40 and its ligand is necessary for amyloid-beta-induced microglial activation, suggesting a role in the early pathogenesis of Alzheimer's disease. Two alternatively spliced transcript variants encoding distinct isoforms of CD40 have been identified. Diseases associated with CD40 dysfunction include Type 3 Hyper-IgM immunodeficiency and CD40 ligand deficiency, highlighting its importance in immune and inflammatory responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD antigen CD40; CD40; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; CDw40; Immunoglobulin M; ImmunoglobulinM; MGC9013; sCD40; soluble CD40; Tumor necrosis factor receptor superfamily member 5
Gene Aliases: Bp50; CD40; CDW40; EGM_02012; p50; TNFRSF5
UniProt ID: (Human) P25942
Entrez Gene ID: (Human) 958, (Cynomolgus monkey) 102118696
positive regulation of protein phosphorylation
immune response-regulating cell surface receptor signaling pathway
protein complex assembly
cellular calcium ion homeostasis
inflammatory response
immune response
multicellular organism development
platelet activation
positive regulation of B cell proliferation
response to lipopolysaccharide
positive regulation of interleukin-12 production
tumor necrosis factor-mediated signaling pathway
B cell proliferation
regulation of cell proliferation
positive regulation of tyrosine phosphorylation of Stat1 protein
defense response to protozoan
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAP kinase activity
protein kinase B signaling
positive regulation of GTPase activity
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgG isotypes
regulation of immune response
regulation of immunoglobulin secretion
positive regulation of NF-kappaB transcription factor activity
defense response to virus
cellular response to lipopolysaccharide
cellular response to mechanical stimulus
positive regulation of protein kinase C signaling
apoptotic signaling pathway
positive regulation of endothelial cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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