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Invitrogen
CD301b (MGL2) Monoclonal Antibody (11A10-B7), eFluor™ 660, eBioscience™
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FIGURE: 1 / 2
CD301b (MGL2) Antibody (50-3011-82) in Flow
Mouse skin draining lymph node cells were fixed with the IC Fixation Buffer (Product # 00-8222-49), then stained with Anti-Mouse CD3e FITC (Product # 11-0031-82), Anti-Mouse CD19 FITC (Product # 11-0193-82), Anti-Mouse MHC Class II (I-A/I-E) eFluor® 450 (Product # 48-5321-82), Anti-Mouse CD11c PE (Product # 12-0114-82), and 0.125 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (left) or 0.125 µg of Anti-Mouse CD301b (MGL2) eFluor® 660 (right). Viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # ... View More
Product Details
50-3011-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, lambda
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
11A10-B7
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574197
Product Specific Information
Description: The monoclonal antibody 11A10-B7 recognizes mouse CD301b. CD301b is also known as macrophage galactose-type C-type lectin 2 (MGL2). CD301b expression is restricted to dermal dendritic cells, connection tissue, and bone marrow derived dendritic cells. Macrophages also express CD301b at low levels. CD301b contains a single carbohydrate recognition domain that recognizes protein antigens that have a galactose and N-acetylgalactosamine (Gal/GalNAc). Gal/GalNAc protein antigens are efficiently internalized through CD301b and presented to antigen specific CD4+ cells via MHC Class II. CD301b+ dendritic cells are believed to play an important role in TH2 priming.
Applications Reported: This 11A10-B7 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 11A10-B7 antibody has been tested by flow cytometric analysis of mouse skin draining lymph node cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD301b is also known as macrophage galactose-type C-type lectin 2 (MGL2). CD301b expression is restricted to dermal dendritic cells, connection tissue, and bone marrow derived dendritic cells. Macrophages also express CD301b at low levels. CD301b contains a single carbohydrate recognition domain that recognizes protein antigens that have a galactose and N-acetylgalactosamine (Gal/GalNAc). Gal/GalNAc protein antigens are efficiently internalized through CD301b and presented to antigen specific CD4+ cells via MHC Class II. CD301b+ dendritic cells are believed to play an important role in TH2 priming.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: CD301; macrophage galactose N-acetyl-galactosamine specific lectin 2; macrophage galactose-type C-type lectin 2
Gene Aliases: CD301b
Entrez Gene ID: (Mouse) 216864
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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