Invitrogen

CD3/HLA Class 2 Antibody Cocktail, FITC, PE

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-16958

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

Neat
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1/IgG2a

Class

Cocktail

Type

Antibody

Clone

UCHT1/WR18

Immunogen

Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome, Human B-CLL cells

Conjugate

FITC , PE FITC , PE FITC , PE

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

purified

Storage buffer

PBS with 1% BSA, 5% sucrose

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538433

Product Specific Information

MA5-16958 contains a monoclonal mixture of FITC-conjugated CD3 (clone #UCH-T1, Mouse IgG1) and RPE-conjugated HLA Class 2 (clone #WR18, Mouse IgG2a). UCHT1 recognizes the human CD3 cell surface antigen expressed by T lymphocytes. WR18 recognizes a common determinant on the beta chain of the human HLA Class 2 molecules HLA-DR, DP and DQ. This antigen is expressed on B lymphocytes, monocytes and on activated T lymphocytes. This antibody is designed to permit the simultaneous enumeration of CD3 and HLA Class 2 expressing cells. It is particularly useful in the assessment of HLA Class 2 expression on T lymphocytes.

Reconstitute with 0.5 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: beta1 domain MHC class II HLA DPB; CD3 antigen, delta subunit; CD3 delta; CD3 epsilon; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD74 antigen; class II HLA beta chain; DHLAG; DW2.2/DR2.2; FLJ18683; HLA class II antigen; HLA class II histocompatibility antigen, DP beta 1 chain; HLA class II histocompatibility antigen, DP(W4) beta chain; HLA class II histocompatibility antigen, DQ beta 1 chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA class II histocompatibility antigen, DRB1 beta chain; HLA DP14-beta chain; HLA-DP histocompatibility type, beta-1 subunit; HLA-DQ; HLA-DR-gamma; HLA-DRB1; HLADG; human leucocyte antigen DRB1; Human leukocyte antigen DRB1; IA-gamma; lymphocyte antigen; lymphocyte antigen DRB1; major histocompatibility complex class II antigen beta chain; MHC class II antigen; MHC class II antigen beta chain; MHC class II antigen DP beta 1 chain; MHC class II antigen DPB1; MHC class II antigen DPbeta1; MHC class II antigen DQB1; MHC class II antigen HLA-DQ-beta-1; MHC class II DQ beta chain; MHC class II HLA-DP-beta-1; MHC class II HLA-DQ beta glycoprotein; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR-beta cell surface glycoprotein; MHC class II HLA-DRB1; MHC class II HLA-DRw10-beta; MHC class2 antigen; MHC DQ beta; MHC HLA DPB1; OKT3, delta chain; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain

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Gene Aliases: CD3-DELTA; CD3-GAMMA; CD3D; CD3E; CD3G; CELIAC1; DPB1; DRB1; DRw10; HLA-DP; HLA-DP1B; HLA-DPB; HLA-DPB1; HLA-DQB; HLA-DQB1; HLA-DR1B; HLA-DRB; HLA-DRB1; IDDM1; IMD17; IMD18; IMD19; SS1; T3D; T3E; T3G; TCRE

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UniProt ID: (Human) P04234, (Human) P07766, (Human) P09693, (Human) P04440, (Human) P01920, (Human) P01911

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Entrez Gene ID: (Human) 915, (Human) 916, (Human) 917, (Human) 3115, (Human) 3119, (Human) 3123

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Function(s)
transcription coactivator activity transmembrane signaling receptor activity protein heterodimerization activity receptor signaling protein activity protein binding SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding peptide antigen binding MHC class II receptor activity MHC class II protein complex binding
Process(es)
positive regulation of T cell anergy protein complex assembly cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway signal complex assembly G-protein coupled receptor signaling pathway response to nutrient positive regulation of gene expression negative regulation of gene expression T cell costimulation positive regulation of interferon-gamma production positive regulation of interleukin-4 production positive regulation of T cell proliferation T cell activation regulation of apoptotic process negative thymic T cell selection positive regulation of interleukin-2 biosynthetic process negative regulation of smoothened signaling pathway positive regulation of alpha-beta T cell proliferation positive regulation of peptidyl-tyrosine phosphorylation regulation of immune response positive regulation of calcium-mediated signaling T cell receptor signaling pathway apoptotic signaling pathway T cell differentiation positive thymic T cell selection positive regulation of transcription from RNA polymerase II promoter antigen processing and presentation of exogenous peptide antigen via MHC class II positive regulation of T cell activation interferon-gamma-mediated signaling pathway polysaccharide assembly with MHC class II protein complex immunoglobulin production involved in immunoglobulin mediated immune response humoral immune response mediated by circulating immunoglobulin immune response establishment or maintenance of cell polarity protein transport Fc-gamma receptor signaling pathway involved in phagocytosis regulation of lymphocyte apoptotic process
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