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Leinco Technologies
CD16.2 Monoclonal Antibody (9 E9), Functional Grade, In Vivo PLATINUM™
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Product Details
C860-100MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
9 E9
Immunogen
Immunizing Armenian hamsters with an Fc-gamma-RIV-IgG1 fusion protein consisting of the extracellular domain of Fc-gamma-RIV fused to a mouse IgG1 Fc portion (D265A-variant deficient in Fc-receptor binding)
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.2-7.4
Contains
no preservative
Storage conditions
4°C short term, -80°C long term
Shipping conditions
Wet ice
Product Specific Information
Specificity: 9E9 activity is primarily directed against mouse CD16.2 / FcgammaRIV but can also bind and block FcgammaRIII in vivo.
Endotoxin Level: <0.5 EU/mg as determined by the LAL method.
Purity: ≥98% monomer by analytical SEC and >95% by SDS Page.
Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing: To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage: Functional grade preclinical antibodies may be stored sterile as received at 2-8C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80C. Avoid Repeated Freeze Thaw Cycles.
Target Information
CD16, known as Fc gamma receptor III, exists in two distinct isoforms: Fc gamma RIIIA and Fc gamma RIIIB. These isoforms are encoded by the genes FCGR3A and FCGR3B, respectively, which are located on chromosome 1 and share a high degree of similarity. Fc gamma RIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as natural killer (NK) cells, but not on T or B cells. This receptor recognizes the Fc portion of immunoglobulin G (IgG) and is involved in the clearance of immune complexes from circulation. Upon binding IgG, Fc gamma RIIIA activates signal transduction pathways that result in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation. Fc gamma RIIIB is expressed on polymorphonuclear neutrophils (PMN) as a glycosylphosphatidylinositol (GPI)-anchored form. It plays a role in similar immune functions, including cellular-mediated cytotoxicity and enhancement of virus infections. Mutations in the FCGR3A gene have been associated with Immunodeficiency 20 and linked to susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD16-2; CD16a; CD32; CD32 receptor 2; CDw32; Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity III, receptor for (CD16); Fc gamma receptor III; Fc gammaRIV; Fc receptor-like 3; Fc-gamma RII-b; Fc-gamma RII-c; fc-gamma RIII; Fc-gamma-RIIb; Fc-gamma-RIIc; Fcg receptor III; FCG2; FcgammaRIII; FcgammaRIV; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcRII-b; FcRII-c; fcRIII; igG Fc receptor III; IgG Fc receptor III-A; low affinity immunoglobulin gamma Fc region receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; RP11-5K23.1
Gene Aliases: 4833442P21Rik; CD16; CD16-2; FcgammaRIV; Fcgr3a; Fcgr4; FcgRIV; Fcrl3
UniProt ID: (Mouse) A0A0B4J1G0
Entrez Gene ID: (Mouse) 14131, (Mouse) 246256
antibody-dependent cellular cytotoxicity
positive regulation of type IIa hypersensitivity
positive regulation of type III hypersensitivity
positive regulation of type I hypersensitivity
serotonin secretion
phagocytosis, recognition
phagocytosis, engulfment
cell surface receptor signaling pathway
neutrophil chemotaxis
Fc-gamma receptor signaling pathway
positive regulation of tumor necrosis factor biosynthetic process
antigen processing and presentation of exogenous peptide antigen via MHC class I
mast cell activation
positive regulation of phagocytosis
regulation of immune response
NK T cell proliferation
regulation of sensory perception of pain
cellular response to lipopolysaccharide
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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