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Invitrogen
CD11a/CD18 Monoclonal Antibody (24)
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Product Details
MA5-33356
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
24
Conjugate
Form
Liquid
Concentration
100 µg/mL
Purification
Protein G
Storage buffer
PBS with 0.1% BSA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815494
Product Specific Information
Product is supplied at 1 mL (100 µL/mL). This antibody inhibits monocyte-dependent, antigen specific T cell proliferation and IL-2-activated natural killer cell assays. The antibody does not interfere with mitogen-stimulated T cell proliferation. Furthermore it prevents deadhesion of receptor/ligand pairs, possible locking leukocyte integrins in an active conformation.
Application Notes: Flow cytometry - This antibody stains the extracellular domain of CD11/CD18. Functional Assay -This antibody acts as an inhibitor. The antibody was functionally tested by inhibition of antigen-specific T cell proliferation and LAK cell cytotoxy, which are LEA-1-dependent, and f-Met-Leu-Phe-stimulated chemotaxis, which is a CR3-dependent process. Positive control: PMN, monocytes, lymphocytes.
Target Information
CD11/CD18 (LFA-1) are beta 2-type integrins. The leukocyte integrins are a family of heterodimeric receptors that mediate divalent cation-dependent cellular adhesion reactions. T cells use integrins in essentially all of their functions. Integrins become active following signalling through other membrane receptors, which cause both affinity alteration and an increase in integrin clustering. The monoclonal antibody 24 recognizes a structural feature and is strictly dependent upon the presence of Mg2+. The epitope is located within, or in close proximity to, the three conserved cation binding domains and therefore a measure of Mg2+ bound to the leukocyte integrins and thus reflects functionally active molecules. The epitope can be induced on polymorphonuclear leukocytes and on monocytes. Glu173 and Glu175 of the beta(2) I domain are identified as critical for antibody 24 binding. The monoclonal antibody 24 inhibits monocyte-dependent, antigen specific T cell proliferation and IL-2- activated natural killer cell assays. The antibody does not interfere with mitogen-stimulated T cell proliferation. Furthermore the monoclonal antibody 24 prevents deadhesion of receptor/ligand pairs, possible locking leukocyte integrins in an active conformation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: antigen CD11A (p180); antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; CD11 antigen-like family member A; CD11a; CD18; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor); Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; complement component 3 receptor 3 and 4 subunit; complement receptor C3 beta-subunit; Complement receptor C3 subunit beta; integrin alpha L; Integrin alpha-L; integrin beta chain, beta 2; Integrin beta-2; integrin gene promoter; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); Leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte cell adhesion molecule CD18; Leukocyte function-associated molecule 1 alpha chain; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; LFA-1 alpha; LFA-1A; lymphocyte function-associated antigen 1; lymphocyte function-associated antigen 1, alpha polypeptide
Gene Aliases: CD11A; CD18; ITGAL; ITGB2; LAD; LCAMB; LFA-1; LFA1A; MAC-1; MF17; MFI7
UniProt ID: (Human) P20701, (Human) P05107
Entrez Gene ID: (Human) 3683, (Human) 3689
leukocyte migration involved in inflammatory response
phagocytosis
apoptotic process
inflammatory response
cell adhesion
leukocyte cell-cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
cell-cell signaling
aging
regulation of cell shape
natural killer cell activation
extracellular matrix organization
neutrophil chemotaxis
receptor internalization
heterotypic cell-cell adhesion
toll-like receptor 4 signaling pathway
endodermal cell differentiation
receptor clustering
endothelial cell migration
cellular extravasation
positive regulation of nitric oxide biosynthetic process
positive regulation of angiogenesis
regulation of peptidyl-tyrosine phosphorylation
regulation of immune response
leukocyte migration
positive regulation of NF-kappaB transcription factor activity
cellular response to low-density lipoprotein particle stimulus
T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
movement of cell or subcellular component
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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