Invitrogen

CA19-9 Monoclonal Antibody (116-NS-19-9)

CA19-9 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 1

CA19-9 Antibody (MA5-12421) in ICC/IF

Immunofluorescence analysis of CA19-9 was performed using 70% confluent log phase SK-OV-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CA19-9 (116-NS-19-9) Mouse Monoclonal Antibody (Product # MA5-12421) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conju... View More

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Product Details

MA5-12421

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:20

Immunocytochemistry (ICC/IF)

1:250

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

116-NS-19-9

Immunogen

Human colon carcinoma cell line SW1116

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10980397

Product Specific Information

MA5-12421 targets CA19-9 in IHC (P) applications and shows reactivity with Human samples.

The MA5-12421 immunogen is human colon carcinoma cell line SW1116.

Target Information

Mucin glycoprotein is a Sialyl Lewis A structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lewis A and/or Lewis B blood group antigens. In normal tissues, sialyl Lewis A antigen is present in ductal epithelium of the breast, kidney, salivary gland, and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas, including adenocarcinomas of the stomach, intestine, and pancreas. Preoperative elevated CA19-9 levels in patients with stage I pancreatic carcinoma decrease to normal values following surgery. When used serially, CA19-9 can predict recurrence of disease prior to radiographic or clinical findings.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CDKN2A-Mutated Pancreatic Ductal Organoids from Induced Pluripotent Stem Cells to Model a Cancer Predisposition Syndrome.

Cancers

Merkle J,Breunig M,Schmid M,Allgöwer C,Krüger J,Melzer MK,Bens S,Siebert R,Perkhofer L,Azoitei N,Seufferlein T,Heller S,Meier M,Müller M,Kleger A,Hohwieler M

MA5-12421 was used in Immunohistochemistry to provide a model for hereditary human pancreatic cancer that enables dissection of tumor initiation and early development starting from patient-specific CDKN2A-mutated pluripotent stem cells.

Wed Oct 13 00:00:00 UTC 2021

Human gastric mucins differently regulate Helicobacter pylori proliferation, gene expression and interactions with host cells.

PloS one

Skoog EC,Sjöling Å,Navabi N,Holgersson J,Lundin SB,Lindén SK

MA5-12421 was used in Enzyme-linked immunosorbent assay to examine the Helicobacter pylori response to purified mucins from a range of tumour and normal human gastric tissue samples.

Fri Sep 21 00:00:00 UTC 2012

Pulmonary meningothelial-like nodules: new insights into a common but poorly understood entity.

The American journal of surgical pathology

Mukhopadhyay S,El-Zammar OA,Katzenstein AL

MA5-12421 was used in immunohistochemistry to study pulmonary meningothelial-like nodules

Wed Apr 01 00:00:00 UTC 2009

Introduction of Sd(a) carbohydrate antigen in gastrointestinal cancer cells eliminates selectin ligands and inhibits metastasis.

Cancer research

Kawamura YI,Kawashima R,Fukunaga R,Hirai K,Toyama-Sorimachi N,Tokuhara M,Shimizu T,Dohi T

MA5-12421 was used in flow cytometry to study the loss of selectin ligands and reduced metastsis in human gastrointestinal tract cancer cells recombinantly expressing Sd(a) carbohydrate antigen

Fri Jul 15 00:00:00 UTC 2005

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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