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Invitrogen
Arginase 1 Polyclonal Antibody
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Arginase 1 Antibody (PA5-22009) in ELISA
Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Arginase 1 Monoclonal Antibody (GT5811) (Product # MA5-31577) as capture antibody at concentration of 5 µg/mL and Arginase 1 Polyclonal Antibody (Product # PA5-22009) as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) was diluted at 1:10,000 and used to detect the primary antibody.
Product Details
PA5-22009
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 322 (P05089) of Arginase I
Conjugate
Form
Liquid
Concentration
1 mg/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
PA5-22009 targets arginase I in ICC, IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples. For immunohistochemistry, antigen retrieval using heat mediated 10 mM citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0) is recommended.
The PA5-22009 immunogen is recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 322 (P05089) of Arginase I.
Target Information
Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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